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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(3): 705-718
DOI: http://dx.doi.org/10.20546/ijcmas.2016.503.083


Role the Employee of Some Biological Stains in Detecting Giardia lamblia among Internal Iraqi Displaced Peoples in Kirkuk Province
Yahya Jirjees Salman1*, Abdul-Rahman Aziz Al-Taee2 and Ali Mohammed Abid3
1College of dentistry, Kirkuk University, Iraq
2College of Medicine, Tikrit University, Iraq
3College of Science, Tikrit University, Iraq
*Corresponding author
Abstract:

Giardiasis is waterborne disease which had impact on public health in anywhere of the world, particularly in communities living under low level of sanitation and poor hygienic condition. Selection of such laboratory methods with high accuracy and precise technique recently had great role in reducing the rate of intestinal parasites in general and Giardia lamblia in particular. So the current study was conducted to fulfill this purpose. Three different microscopic based laboratory techniques namely  direct double wet preparation, formal-ether and fecal stained smears by 9 different biological stains were applied for detecting Giardia lamblia stages in a total of 417 stool samples of Internal Iraqi Displaced peoples(IIDPPs) in Kirkuk city. The overall rate of Intestinal parasitic infection was 19.66%(82),,which included 10.31 %(43) as pure giardiasis and 9.35 %(39) for other 9 intestinal parasites. They involve: Blastocyst homonis 4.17 %, Entamoeba histolytica 1.67 %,Cryptosporidium parvum 1.43 %, Entamoeba coli 0.71 %, Cyclospora cayetanensis, 0.49 %, and 0.23 % for each of Entamoeba hartmani, Iodomoeba butschili, Hymenolepis nana and Ancylostoma duodenale. Statistically formalin-ether technique show high efficacy and significance than wet preparation technique in demonstrating other intestinal parasites. Eighty two positive samples were tested as fecal smears stained by nine different biological stains were examined by microscope. High rates of giardiasis 36.58 %.34.14 %,32.92 % and 25.6 % were recorded by using alcoholic eosin 1%, modified Gram staining+chromotrope 2R, alcoholic aceto-carmine 1 % and calcofluor white stains respectively. Followed by 24.39 %, 23.17 % and 19.15 % for Chromotrope 2R,trichrome stain and modified Ziehl-Neelsen stains respectively. While low rates 10.97 % and 7.31 % were recorded for haematoxyline-Ehrlich and 3 % of giemsa stain respectively. Giardiasis was contributing high rate from intestinal parasites among IIDPs,which had high healthy  impact of this group of Iraqi communities. Fecal smears staining methods show high efficacy than direct microscopy and sedimentation technique in detecting and demonstrating Giardia lamblia microscopically, specially 1% of alcoholic eosin, alcoholic aceto-carmine and modified gram staining-hot chromotrope 2R stains.


Keywords: Giardiasis, formal-ether, Calcofluor, Chromotrope 2R, Giemsa.
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How to cite this article:

Yahya Jirjees Salman, Abdul-Rahman Aziz Al-Taee and Ali Mohammed    Abid. 2016. Role the Employee of Some Biological Stains in Detecting Giardia lamblia among Internal Iraqi Displaced Peoples in Kirkuk Province.Int.J.Curr.Microbiol.App.Sci. 5(3): 705-718. doi: http://dx.doi.org/10.20546/ijcmas.2016.503.083