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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 2523-2530
DOI: https://doi.org/10.20546/ijcmas.2017.605.283


Use of RAPD Marker for the Assessment of Genetic Diversity of Sesame (Sesamum indicum L.) Varieties
Kanak Saxena* and Rajani Bisen
Department of Genetics and Plant Breeding, College of Agriculture, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur (M.P.), India
*Corresponding author
Abstract:

Genetic diversity among 28 sesame (Sesamum indicum L.) varieties was examined at DNA level by means of random amplified polymorphic DNA (RAPD) analysis. Fifteen primers used produced a total of 132 RAPD fragments. Each primer generated 3 to 26 amplified fragments with an average of 8.8 bands per primer. Based on pair-wise comparisons of RAPD amplification products, Nei and Li’s similarity coefficients were computed to assess the associations among the varieties. Pair-wise similarity indices varied from 0.35 to 0.84. A UPGMA cluster analysis based on these genetic similarities located most of the varieties far apart from one another, showing a high level of polymorphism. Genetically, all the genotypes were classified into two major groups at similarity coefficient 0.35 and these major groups are further divided into small clusters as the similarity coefficient level increases. Accession Swetha Til and AKT-101 was show maximum distance from each other. In conclusion, even with the use of a limited set of primers, RAPD technique revealed a high level of genetic variation among sesame varieties. This high level of genetic diversity among the genotypes suggested that RAPD technique is valuable for sesame systematic, and can be helpful for competent choice of parents in breeding programs.


Keywords: RAPD, Genetic diversity, Polymorphism
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How to cite this article:

Kanak Saxena and Rajani Bisen. 2017. Use of RAPD Marker for the assessment of genetic diversity of sesame (Sesamum indicum L.) varieties.Int.J.Curr.Microbiol.App.Sci. 6(5): 2523-2530. doi: https://doi.org/10.20546/ijcmas.2017.605.283