National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The gastrointestinal tract of fish represents an important reservoir of microorganisms adapted to the host environment and therefore provides a useful source for the selection of autochthonous probiotic candidates. The present study aimed to isolate lactic acid bacteria from the gut of healthy salmon fish, screen the isolates for probiotic-associated traits, and identify the most promising isolate by 16S rRNA gene sequencing. Gut material was aseptically collected, homogenized and serially diluted before plating on de Man, Rogosa and Sharpe (MRS) agar. Four morphologically distinct isolates, designated C1–C4, were recovered. Isolate C1 was selected after preliminary characterization because it was a Gram-positive rod and showed negative catalase and oxidase reactions. The isolate tolerated exposure to pH 2.5 and showed moderate growth in the presence of 0.3% bile salts. It also exhibited γ-haemolysis on blood agar. Cell-free supernatant of C1 inhibited Salmonella Typhi, Escherichia coli, Proteus vulgaris and Acinetobacter baumannii, with inhibition zones of 18.0, 13.5, 13.0 and 10.5 mm, respectively. Molecular characterization generated an approximately 1,500-bp 16S rRNA amplicon. BLAST comparison showed 99.6% identity with Lactobacillus plantarum strain ATCC 14917, 99.4% with strain WCFS1 and 99.2% with strain JDM1, and the phylogenetic analysis placed isolate L. plantarum clade. The isolate therefore showed a combination of gastrointestinal stress tolerance, non-haemolytic phenotype and antagonistic activity that supports its consideration as a potential fish-feed probiotic.
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