National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Marine backwater sediments contain diverse cultivable microorganisms that may produce metabolites with useful biological activities. This study evaluated bacteria isolated from Muttukadu backwaters, Tamil Nadu, India, for free-radical-scavenging activity and preliminary cytotoxicity. Three sediment samples yielded twelve bacterial isolates, and ethyl acetate extracts of culture broths were examined using the DPPH assay. Four isolates were selected for secondary screening, of which isolate B4 showed the highest activity (49.52 ± 0.71%) and was taken forward for optimization and characterization. Variation in culture conditions altered the antioxidant response, and the optimized extract showed a concentration-related increase in DPPH scavenging, reaching 45.29 ± 0.97% at 0.5 mg/mL. TLC separation produced seven visible components, and the fraction at Rf 0.71 showed positive DPPH bioautography. Phenotypic and biochemical testing indicated a Gram-negative, rod-shaped, non-motile bacterium, while partial 16S rRNA analysis showed the closest relationship to Alcaligenes faecalis with 99% sequence similarity. The crude extract also decreased HeLa cell viability in a concentration-dependent manner, with an IC50 of 65.14 µg/mL. The findings demonstrate antioxidant activity in a cultivable bacterium from Muttukadu backwaters and identify an activity-positive extract that warrants further purification and chemical characterization.
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