National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The emergence of antimicrobial resistance necessitates the search for new bioactive molecules, particularly those derived from local plant resources. This study, conducted at the Veterinary Diagnostic Laboratory of Brazzaville (LDVB), evaluates the antimicrobial activity of Tetracarpidium conophorum seed oil collected in Lékana, Republic of Congo, against three bacterial strains (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae) and one fungal strain (Candida albicans). The protocol relied on agar well diffusion using blank discs impregnated with oil and antibiotic controls, as well as the determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) in 96-well microplates using p-iodonitrotetrazolium chloride as a colorimetric indicator. The results highlight a targeted and statistically significant inhibitory activity (p < 0.05): the oil proved particularly effective against Gram-negative pathogens (K. pneumoniae and E. coli), while no inhibition was observed for S. aureus and C. albicans. Klebsiella pneumoniae was more sensitive than E. coli, with increased sensitivity for the LDVB isolate. These data suggest that T. conophorum oil holds promising potential as a natural antibacterial agent, offering interesting prospects for biological risk management and the health safety of foods of animal origin.
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