National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Microalgae have emerged as promising biological resources owing to their high nutritional value, rapid growth, and diverse biotechnological applications. The present study aimed to evaluate the effect of superphosphate supplementation on the growth and biochemical composition of Chlorella vulgaris isolated from Poompozhil Lake, Thiruvallur District, Tamil Nadu. The algal culture was maintained under controlled laboratory conditions using dechlorinated tap water at 20°C with a 12 h light:12 h dark photoperiod. Superphosphate was employed as a nutrient supplement in the treatment group, while untreated cultures served as controls. Growth performance was assessed by hemocytometer-based cell counts, and biochemical constituents including carbohydrates, proteins, and lipids were estimated using standard analytical methods such as Anthrone, Lowry, and Bligh and Dyer techniques. The results demonstrated a significant enhancement in algal growth under superphosphate treatment. Cell density increased progressively from 472 × 10⁴ to 770 × 10⁴ cells mL⁻¹ in treated cultures, compared to considerably lower counts in control conditions. The treated cultures also exhibited improved pH conditions and enhanced biomass accumulation. Biochemical analysis revealed increased concentrations of major cellular components, including carbohydrates (0.9938 mg g⁻¹), proteins (6.56 mg g⁻¹), and lipids (0.3278 mg g⁻¹) compared with control cultures. The findings indicate that superphosphate supplementation effectively stimulates cellular division, biomass production, and metabolite accumulation in Chlorella vulgaris. The study highlights a cost-effective approach for large-scale algal cultivation and suggests potential applications in biomass production, biofertilizer recycling, nutraceutical development, and sustainable biotechnology.
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