National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Trichoderma species have emerged as powerful biocontrol agents for controlling various diseases of crops and plants. Fungi belonging to Diaporthe and Lasiodiplodia species comprise some highly destructive plant pathogens affecting important agricultural crops. In the present study, the Trichoderma isolates were assessed for their antagonistic potential against Diaporthe and Lasiodiplodia species. The two methods used were dual culture assay and inverted plate assay. In the dual culture assay, the percentage inhibition of radial growth (PIRG) of Diaporthe species ranged from 65.16% to 73.68% with the maximum PIRG shown by isolate T4, which was identified as Trichoderma erinaceum; and the PIRG of Lasiodiplodia species ranged from 57.14% to 62.04% with the maximum PIRG shown by isolate T1, which was identified as Trichoderma yunnanense. In the inverted plate assay, the percentage of inhibition of Diaporthe species ranged from 19.79% to 60.29% and the maximum inhibition was exhibited by isolate T4. The volatile compounds produced by the Trichoderma isolates showed no inhibitory effect on the growth of Lasiodiplodia species. Thus, it can be concluded that the Trichoderma isolates showed more effective antagonism against Diaporthe than against Lasiodiplodia.
Abdul-Halim, A.M., Shivanand, P., Krishnamoorthy, S. and Taha, H., 2023. A review on the biological properties of Trichoderma spp. as a prospective biocontrol agent and biofertilizer. Journal of Applied Biology & Biotechnology, 11(5), pp. 34-46. https://doi.org/10.7324/JABB.2023.11504
Alshammari, W., Bairum, R., Sulieman, A.M., Alshammari, N. and Elamin, H., 2024. In vitro and in vivo study of antagonistic and biocontrol of Trichoderma harzianum strains against wood decay pathogens. Polish Journal of Environmental Studies, 33(1), pp.515-521. https://doi.org/10.15244/pjoes/172043
Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J., 1990. Basic local alignment search tool. Journal of molecular biology, 215(3), pp.403-410. https://doi.org/10.1016/S0022-2836(05)80360-2
Anastasiadis, I.A., Giannakou, I.O., Prophetou-Athanasiadou, D.A. and Gowen, S.R., 2008. The combined effect of the application of a biocontrol agent Paecilomyces lilacinus, with various practices for the control of root-knot nematodes. Crop Protection, 27(3-5), pp.352-361. https://doi.org/10.1016/j.cropro.2007.06.008
Aneja, K.R., 2007. Experiments in microbiology, plant pathology and biotechnology. New Delhi: New Age International.
Atallah, O.O., Hassanin, A.A., Yassin, S.M., Aloufi, A.S., Almanzalawi, E.A., Abdelkhalek, A., Atia, M.M., Behiry, S., Abdelrhim, A.S. and Nehela, Y., 2024. Pathological characterization and management of Lasiodiplodia theobromae, a hemibiotroph with an interkingdom host range. Plant Disease, 108(11), pp.3243-3257. https://doi.org/10.1094/PDIS-03-24-0713-RE
Ayaz, M., Li, C.H., Ali, Q., Zhao, W., Chi, Y.K., Shafiq, M., Ali, F., Yu, X.Y., Yu, Q., Zhao, J.T. and Yu, J.W., 2023. Bacterial and fungal biocontrol agents for plant disease protection: Journey from lab to field, current status, challenges, and global perspectives. Molecules, 28(18), p.6735. https://doi.org/10.3390/molecules28186735
Barnett, H.L. and Hunter, B.B., 1972. Illustrated genera of imperfect fungi. USA: APS Press.
Begum, M.M., Sariah, M., Abidin, M.Z., Puteh, A.B. and Rahman, M.A., 2008. Ultrastructural studies of soybean seed-borne infection by Diaporthe phaseolorum var. sojae and screening of antagonistic potentiality by selected biocontrol agents in vitro. Pertanika J. Trop. Agric. Sci, 31(2), pp.247-256.
Bhadra, M., Khair, A., Hossain, M.A. and Sikder, M.M., 2014. Efficacy of Trichoderma spp. and fungicides against Lasiodiplodia theobromae. Bangladesh Journal of Scientific and Industrial Research, 49(2), pp.125-130. https://doi.org/10.3329/bjsir.v49i2.22008
Billar de Almeida, A., Concas, J., Campos, M.D., Materatski, P., Varanda, C., Patanita, M., Murolo, S., Romanazzi, G. and Felix, M.D.R., 2020. Endophytic fungi as potential biological control agents against grapevine trunk diseases in Alentejo region. Biology, 9(12), p.420. https://doi.org/10.3390/biology9120420
da Silva França, K.R., de Medeiros Ferro, M.M., Da Silva, M.V.S., Ramos-Sobrinho, R., De Melo, M.P., de Andrade Lima, G.S. and Assunção, I.P., 2025. Diversity and pathogenicity of Lasiodiplodia species associated with banana crown rot in Northern and Northeastern Brazil. Scientific Reports, 15(1), p.38802. https://doi.org/10.1038/s41598-025-22739-z
Dennis, C. and Webster, J., 1971. Antagonistic properties of species-groups of Trichoderma: II. Production of volatile antibiotics. Transactions of the British Mycological Society, 57(1), pp.41-48, IN4. https://doi.org/10.1016/S0007-1536(71)80078-5
El-Ganainy, S.M., Ismail, A.M., Iqbal, Z., Elshewy, E.S., Alhudaib, K.A., Almaghasla, M.I. and Magistà, D., 2022. Diversity among Lasiodiplodia species causing dieback, root rot and leaf spot on fruit trees in Egypt, and a description of Lasiodiplodia newvalleyensis sp. nov. Journal of Fungi, 8(11), p.1203.https://doi.org/10.3390/jof8111203
Gams, W. and Bissett, J., 2002. Morphology and identification of Trichoderma. In: Kubicek, C.P. and Harman, G.E., editors. Trichoderma and Gliocladium. Volume 1: Basic biology, taxonomy and genetics (1st edition), London: CRC Press, pp.3-34. https://doi.org/10.1201/9781482295320
George, M., Sureshbabu, B.M., Manoj, A., Belufi, L.M.D.R., de Mello, F.E., Diniz, J.M., Hosseini, B., Infantino, A., Link, T., Marques, D. and Mathioni, S.M., 2025. A diagnostic guide of diseases caused by Diaporthe species in soybean. Plant Health Progress, 26(4), pp.705-716. https://doi.org/10.1094/PHP-03-25-0096-DG
Gomes, R.R., Glienke, C., Videira, S.I.R., Lombard, L., Groenewald, J.Z. and Crous, P.W., 2013. Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. Persoonia-Molecular Phylogeny and Evolution of Fungi, 31(1), pp.1-41. https://doi.org/10.3767/003158513X666844
Hashimi, M.H., Hashimi, R. and Ryan, Q., 2020. Toxic effects of pesticides on humans, plants, animals, pollinators and beneficial organisms. Asian Plant Res. J, 5(4), pp.37-47. https://doi.org/10.9734/aprj/2020/v5i430114
He, C., Lin, J., Wu, H., Zheng, J., Zhang, Y., Zhang, Y., Li, Z., Liang, Y., Lu, Y., Yi, K. and Wu, W., 2025. Morphological and molecular characterization of Lasiodiplodia theobromae causing stem gummosis disease in rubber trees and its chemical control strategies. Microorganisms, 13(7), p.1586. https://doi.org/10.3390/microorganisms13071586
Joo, J.H. and Hussein, K.A., 2022. Biological control and plant growth promotion properties of volatile organic compound-producing antagonistic Trichoderma spp. Frontiers in plant science, 13, p.897668. https://doi.org/10.3389/fpls.2022.897668
Kashyap, U., Garg, S. and Arora, P., 2024. Pesticide pollution in India: environmental and health risks, and policy challenges. Toxicology Reports, 13, p.101801. https://doi.org/10.1016/j.toxrep.2024.101801
Kavitha, B. and Ranganayakulu, G.S., 2025. Isolation and identification of fungi from spoiled vegetables and fruits of Rythubazar, Kurnool, Andhra Pradesh, India. Current Agriculture Research Journal, 13(2), pp. 534-540. http://dx.doi.org/10.12944/CARJ.13.2.12
Kole, R.K., Roy, K., Panja, B.N., Sankarganesh, E., Mandal, T. and Worede, R.E., 2019. Use of pesticides in agriculture and emergence of resistant pests. Indian Journal of Animal Health, 58(2), pp.53-70.
Kumar, V., Koul, B., Taak, P., Yadav, D. and Song, M., 2023. Journey of Trichoderma from pilot scale to mass production: A review. Agriculture, 13(10), p.2022. https://doi.org/10.3390/agriculture13102022
Li, X., Leng, J., Yu, L., Bai, H., Li, X., Wisniewski, M., Liu, J. and Sui, Y., 2022. Efficacy of the biocontrol agent Trichoderma hamatum against Lasiodiplodia theobromae on macadamia. Frontiers in microbiology, 13, p.994422. https://doi.org/10.3389/fmicb.2022.994422
Liang, Z., Ali, Q., Wu, H., Gu, Q., Liu, X., Sun, H. and Gao, X., 2025. Biocontrol mechanism of Bacillus thuringiensis GBAC46 against diseases and pests caused by Fusarium verticillioides and Spodoptera frugiperda. Biomolecules, 15(4), p.519. https://doi.org/10.3390/biom15040519
Marraschi, R., Ferreira, A.B.M., da Silva Bueno, R.N., Leite, J.A., Lucon, C.M.M., Harakava, R., Leite, L.G., Padovani, C.R. and Bueno, C.J., 2019. A protocol for selection of Trichoderma spp. to protect grapevine pruning wounds against Lasiodiplodia theobromae. Brazilian Journal of Microbiology, 50(1), pp.213-221. https://doi.org/10.1007/s42770-018-0029-y
McKeen, C.D., 1957. Phomopsis black rot of cucurbits. Canadian Journal of Botany, 35(1), pp.43-50. https://doi.org/10.1139/b57-008
Morton, D.J. and Stroube, W.H., 1955. Antagonistic and stimulatory effects of microorganisms upon Sclerotium rolfsii. Phytopathology, 45(8), pp.417-420.
Moya, P. A., Girotti, J. R., Toledo, A. V., and Sisterna, M. N. (2018). Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch. Journal of Plant Protection Research, 58(1), pp.45–53. https://doi.org/10.24425/119115
Nagamani, P., Bhagat, S., Biswas, M.K. and Viswanath, K., 2017. Effect of volatile and non- volatile compounds of Trichoderma spp. against soil borne diseases of chickpea. International Journal of Current Microbiology and Applied Sciences, 6(7), pp.1486-1491. https://doi.org/10.20546/ijcmas.2017.607.177
Nassary, E.K., 2025. Fungal biocontrol agents in the management of soil-borne pathogens, insect pests, and nematodes: Mechanisms and implications for sustainable agriculture. The Microbe, 7, p.100391. https://doi.org/10.1016/j.microb.2025.100391
Rabuske, J.E., Muniz, M.F.B., Brun, T., Saldanha, M.A., Sarzi, J.S., Savian, L.G., Walker, C., Rolim, J.M., Zabot, G.L. and Mazutti, M.A., 2023. Trichoderma asperellum in the biocontrol of Lasiodiplodia theobromae and Pseudofusicoccum kimberleyense. Journal of Plant Protection Research, pp.488-498. https://doi.org/10.24425/jppr.2023.147832
Rao, V.G., Kireeti, A., Anoosha, V., Sumitha, S., Jerard, B.A., Neeraja, B. and Mutyalanaidu, M., 2025. Lasiodiplodia theobromae: a facultative pathogen of coconut-a comprehensive review. Plant Archives, 25(2), pp. 1257-1268. https://doi.org/10.51470/PLANTARCHIVES.2025.v25.no.2.181
Singh, P. and Sharma, M., 2020. Cultural and morphological characterization of antagonistic Trichoderma isolates. International Journal of Current Microbiology and Applied Sciences, 9(3), pp.1041-1048. https://doi.org/10.20546/ijcmas.2020.903.122
Thambugala, K.M., Daranagama, D.A., Phillips, A.J., Kannangara, S.D. and Promputtha, I., 2020. Fungi vs. fungi in biocontrol: An overview of fungal antagonists applied against fungal plant pathogens. Frontiers in cellular and infection microbiology, 10, p.604923. https://doi.org/10.3389/fcimb.2020.604923
Thilagam, R., Kalaivani, G. and Hemalatha, N., 2018. Isolation and identification of phytopathogenic fungi from infected plant parts. International Journal of Current Pharmaceutical Research, 10(1), pp.26-28.
http://dx.doi.org/10.22159/ijcpr.2018v10i1.24404
Udayanga, D., Liu, X., McKenzie, E.H., Chukeatirote, E., Bahkali, A.H. and Hyde, K.D., 2011. The genus Phomopsis: biology, applications, species concepts and names of common phytopathogens. Fungal Diversity, 50(1), pp.189-225. https://doi.org/10.1007/s13225-011-0126-9
Vincent, J.M., 1947. Distortion of fungal hyphae in the presence of certain inhibitors. Nature, 159(4051), pp.850-850. https://doi.org/10.1038/159850b0
White, T.J., Bruns, T., Lee, S.J.W.T. and Taylor, J., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications, 18(1), pp.315-322.
Xie, J., Singh, P., Qi, Y., Singh, R.K., Qin, Q., Jin, C., Wang, B. and Fang, W., 2023. Pseudomonas aeruginosa strain 91: a multifaceted biocontrol agent against banana Fusarium wilt. Journal of Fungi, 9(11), p.1047. https://doi.org/10.3390/jof9111047
Yao, X., Guo, H., Zhang, K., Zhao, M., Ruan, J. and Chen, J., 2023. Trichoderma and its role in biological control of plant fungal and nematode disease. Frontiers in microbiology, 14, p.1160551. https://doi.org/10.3389/fmicb.2023.1160551
Zambelli, A., Mancebo, M.F., Bazzalo, M.E., Reid, R.J., Sanchez, M.C., Kontz, B.J. and Mathew, F.M., 2021. Six species of Diaporthe associated with Phomopsis stem canker of sunflower in southern Pampean region of Argentina. Plant Health Progress, 22(2), pp.136-142. https://doi.org/10.1094/PHP-07-20-0059-S
Zhao, L., Zhang, L., Ding, Y., Li, M. and Zhang, Y., 2025. Diaporthe species (Sordariomycetes, Diaporthales) causing walnut blight and dieback in China. MycoKeys, 122, pp.197-221. https://doi.org/10.3897/mycokeys.122.158807|
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