National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Microbial degradation of pesticide residues represents an environmentally sustainable approach for mitigating agrochemical pollution. Prolonged and intensive use of neonicotinoid insecticides such as acetamiprid has resulted in their accumulation in agricultural soils and contamination of surface and groundwater, posing ecological and public health concerns. The present study aimed to isolate and characterize indigenous bacteria capable of degrading acetamiprid from pesticide-contaminated agricultural soil. Selective enrichment culture using mineral salt medium supplemented with acetamiprid (50 mg L⁻¹) led to the isolation of an efficient degrading strain. Morphological and biochemical characterization followed by 16S rRNA gene sequencing identified the isolate as Pantoea eucalypti PD07, a Gram-negative rod-shaped bacterium. Biodegradation of acetamiprid was monitored using UV–visible spectrophotometry, which revealed complete degradation within seven days under shaking conditions at 30 °C. Fourier transform infrared spectroscopy indicated structural transformation of the parent compound, with the formation of the intermediate metabolite N-methyl-(6-chloro-3-pyridyl) methylamine, suggesting enzymatic degradation pathways. Kinetic analysis demonstrated pseudo-first-order degradation behavior with a half-life of approximately 2.1 days. The findings highlight the metabolic versatility of P. eucalypti PD07 and its potential application in the bioremediation of acetamiprid-contaminated agricultural environments.
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