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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2023: 95.56
Index Copernicus ICI Journals Master List 2023 - IJCMAS--ICV 2023: 95.56
For more details click here
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Original Research Articles Volume : 15, Issue : 5, May, 2026

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Int.J.Curr.Microbiol.App.Sci.2026.15(5) : 236-239
DOI : https://doi.org/10.20546/ijcmas.2026.1505.029


Microbial Identification Avian Pathogenic Escherichia coli (APEC) Isolated from Poultry by MALDI-TOF MS Method

Dushyant Madhukarrao1*, Pravin Rahi2 and I. H. Kalyani1

1Department of Veterinary Microbiology, College of Veterinary Science and A.H., Kamdhenu University, Navsari- 396450 (Gujarat), India
2National Centre for Microbial Resource Pune, India


*Corresponding author
Abstract:

To overcome the problems associated with identifying bacteria. The matrix-assisted laser desorption ionization (MALDI) technology was co invented by Franz Hillenkamp and Michael Karas in the mid-1980s. In this work, 77 isolates of avian pathogenic E. coli were analyzed by the MALDI-TOF MS method. Total 77 bacterial isolates were identified as E. coli on the basis on their mass to charge ratio (m/z). The strain showing ≥1.7 log value with strain in database were confirmed as the member of that genus and strains showing ≥2.0 log values were confirmed as the member of that species. Good quality MALDI-TOF MS spectra were generated for all E. coli strains in this study.


Keywords: APEC, EXPEC, E.coli, PCR, MALDITOF

References:

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How to cite this article:

Dushyant Madhukarrao, Pravin Rahi and Kalyani I. H. 2026. Microbial Identification Avian Pathogenic Escherichia coli (APEC) Isolated from Poultry by MALDI-TOF MS Method. Int.J.Curr.Microbiol.App.Sci. 15(5): 236-239 doi: https://doi.org/10.20546/ijcmas.2026.1505.029
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license

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