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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles Volume : 15, Issue : 5, May, 2026

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2026.15(5) : 95-103
DOI : https://doi.org/10.20546/ijcmas.2026.1505.013


Molecular Assessment of Abiotic Stress Tolerance in Selected Inula Species Using PCR-Based Markers

Ermatova Gulzoda Zakirdjanovna and Sharobiddinov Dilshod Azamatjonovich*
Andijan state university, Andijan, Uzbekistan
*Corresponding author
Abstract:

Abiotic stresses such as drought, salinity, and cold are major limiting factors for plant growth and productivity, particularly under the increasing pressures of climate change. This study investigates the molecular basis of stress tolerance in three Inula species—Inula helenium, Inula grandis, and Inula glauca—collected from diverse ecological zones in Uzbekistan. Genomic DNA was extracted using both CTAB and commercial kit-based methods, with the latter yielding higher quality and concentration. Sixteen stress-responsive gene markers associated with drought (e.g., NCED1, POD1), salinity (e.g., NHX2, PP2C8), and cold (e.g., CSP3) were amplified by PCR to evaluate gene presence and expression intensity. I. helenium showed the broadest tolerance, with amplification of 14 out of 16 markers, indicating strong generalist adaptability. I. grandis displayed high drought and salinity responses but lacked cold tolerance, while I. glauca exhibited moderate expression with an emphasis on cold response. These expression patterns align with the ecological distributions of the species and underscore genotype–environment interactions. The identified markers provide valuable tools for future conservation and marker-assisted breeding, with I. helenium emerging as a promising donor of multi-stress tolerance traits for crop improvement under environmental stress conditions.


Keywords: Inula helenium; Inula grandis; Inula glauca; abiotic stress; drought tolerance; salinity tolerance; cold tolerance; ABA signaling; stress-responsive genes; Central Asia


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How to cite this article:

Ermatova Gulzoda Zakirdjanovna and Sharobiddinov Dilshod Azamatjonovich. 2026. Molecular Assessment of Abiotic Stress Tolerance in Selected Inula Species Using PCR-Based Markers. Int.J.Curr.Microbiol.App.Sci. 15(5): 95-103 doi: https://doi.org/10.20546/ijcmas.2026.1505.013
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license

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