National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Quorum sensing (QS) is a system of intercellular communication and regulation of the transcription of resistance, virulence, and pathogenicity genes. The aim of this study was to identify genetic markers controlling quorum sensing in multidrug-resistant P. aeruginosa and E. coli. A set of fifty (50) strains, composed of P. aeruginosa (30) and E. coli (20), were isolated from animal products. These strains underwent phenotypic and biochemical identification. Antibiotic resistance profiles were determined by the Muller-Hinton agar diffusion method. Quorum sensing markers (LasI/LasR) and (RhlI/RhlR) were detected by PCR. The prevalence of the Las gene (LasI/LasR) and the Rhl gene (RhlI/RhlR) was 80% and 60%, respectively, in P. aeruginosa. In E. coli, the prevalence of QS genes was 40% for Las (LasI/LasR) and 40% for Rhl (RhlI/RhlR). The total prevalence of the Las gene and the Rhl gene was 64% (LasI/LasR) and 68% (RhlI/RhlR), respectively, in the studied strains. E. coli strains exhibited penicillin resistance exceeding 25% for amoxicillin (67.5%), amoxicillin-clavulanic acid (46.6%), and piperacillin (28.5%). This resistance was less than 25% for ciprofloxacin (23.7%), ceftazidime (18.6%), cefoxitin (17.8%), cefepime (14.3%), and imipenem (14.6%). P. aeruginosa strains expressed multidrug resistance to ticarcillin (54%), aztreonam (47%), ticarcillin-clavulanic acid (32%), piperacillin (29%), levofloxacin (24%), and ciprofloxacin (29%). The detection and control of genetic factors influencing quorum sensing in multidrug-resistant bacteria can improve diagnosis and contribute to the fight against biofilm infections.
Al-Kilabi, A. A. K., Al-Turaihi, T. S., & Al-Mohammed, H. S. (2020). Molecular detection of quorum sensing genes in Pseudomonas aeruginosa isolated from CSOM patients and their relationship to biofilm ability. EurAsian Journal of BioSciences, 14: 4929-4934.
Ayman, E., Eman M., Husam, M., Edrees, M. I., Safiyah, A., Sulaiman, A., Hussain, A., Abdulaziz, A., Ahmed, A., Mahmoud, J., & Akram, A-O. (2026). Understanding Pseudomonas aeruginosa Biofilms: Quorum Sensing, c-di-GMP Signaling, and Emerging Antibiofilm Approaches. Microorganisms, 14: 109.
Benie, C. K. D., Attien, P. Y., Toe, E., Tra-Bi, Y. C., Atobla. K., & Dadié, A. (2021). Biofilm Formation and Inhibitory Effect of Essential Oils in Multidrug-Resistant Pseudomonas aeruginosa. Journal of Bacteriology and Mycology. 8: 1192.
CA-SFM/EUCAST, (2023). European Committee on Antimicrobial Susceptibility Testing and Antibiogram. Committee of the French Society of Microbiology, 2024, p1-158 p.
Chu, X., & Yang, Q. (2024). Regulatory Mechanisms and Physiological Impacts of Quorum Sensing in Gram-Negative Bacteria. Infection and Drug Resistance. 17: 5395–5410. 10.2147/IDR.S451234
CostaLima, J., Alves, L., Jacomé, P., Neto, J., & VieiraMaciel, M. (2018). Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing, The Brazilian Journal of Infectious Diseases, 22: 129-136. https://doi.org/10.1016/j.bjid.2017.10.005
Dekimpe, V., & Déziel, E., (2009). Revisiting the quorum-sensing hierarchy in Pseudomonas aeruginosa: the transcriptional regulator RhlR regulates Las Rspecific factors. Microbiology, 155: 712–723. https://doi.org/10.1099/mic.0.024273-0
Déziel, E., Lépine, F., Milot, S., He, J., Mindrinos, M. N., Tompkins R.G., & Rahme L. G. (2004). Analysis of Pseudomonas aeruginosa 4-hydroxy-2-alkylquinolines (HAQs) reveals a role for 4-hydroxy-2-heptylquinoline in cell-to-cell communication. Proceedings of the National Academy of Sciences, U S A, 101: 1339–1344. https://doi.org/10.1073/pnas.0307694100
Kumar, A., & Schweizer, H. P. (2005). Bacterial resistance to antibiotics: active efflux and reduced uptake. Advanced Drug Delivery Research, 57: 1486-1513. https://doi.org/10.1016/j.addr.2005.04.004
Maisuria, V. B., Santos, Y. L., Tufenkji, N., & Déziel E. (2016). Cranberry-derived proanthocyanidins impair virulence and inhibit Quorum sensing of Pseudomonas aeruginosa. Scientific Reports, 6: 30-169. https://doi.org/10.1038/srep30169
Papaneophytou, C. (2026). Phytochemical Quorum-Sensing Inhibitors Against Bacterial Pathogens: Mechanisms of Action and Translational Challenges, Curr. Issues Mol. Biol. 48: 214.
Pournajaf, A., Razavi, S., Irajian, G., Ardebili, A., Erfani, Y., & Solgi, S. (2018). Integron types, antimicrobial resistance genes, virulence gene profile, alginate production and biofilm formation in Iranian cystic fibrosis Pseudomonas aeruginosa isolates. Le infezioni in medicina, 26: 226-236.
Pumbwe, L., Skilbeck, C. A., & Wexler, H. M. (2008). “Presence of Quorum?Sensing Systems Associated With Multidrug Resistance and Biofilm Formation in Bacteroides fragilis.” Microbial Ecology, 3: 412–419. https://doi.org/10.1007/s00248-007-9295-3
Qin, S., Xiao, W., Zhou, C., Pu, Q., Deng, X., & Lan, L. (2022). Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics. Signal Transduct. Target. Ther. 7:199. https://doi.org/10.1038/s41392-022-00930-7
Qu, Y. Y., Zou, G., Wang, Y., Zhang, & Q. Yu. (2024). “Disruption of Communication: Recent Advances in Antibiofilm Materials With Anti?Quorum Sensing Properties.” ACS Applied Materials & Interfaces 16, no. 11: 13353–13383. https://doi.org/10.1021/acsami.3c18015
Rasamiravaka, T., Labtani Q., Duez P., & El J. M. (2015). The formation of biofilms by Pseudomonas aeruginosa: a review of the natural and synthetic compounds interfering with control mechanisms. BioMed Research International, 2015: 1-17. https://doi.org/10.1155/2015/759348
Rather, M. A., Gupta, K., & Mandal, M. (2021). Inhibition of biofilm and quorum sensing-regulated virulence factors in Pseudomonas aeruginosa by Cuphea carthagenensis (Jacq.) J. F. Macbr. Leaf extract: an in vitro study. J. Ethnopharmacol. 269:113699. https://doi.org/10.1016/j.jep.2020.113699
Raya, J., Montagut, E-J., & Marco, M-P. (2025). Analysing the integrated quorum sensing (iqs) system and its potential role in Pseudomonas aeruginosa pathogenesis. Front. Cell. Infect. Microbiol. 15:1575421.
Rosignoli, S., Elisa, L., Olga, S., Serena, R., Elisabetta, R., Alessandro, P. & Ivana C. (2026). Bioinformatics-Driven, Plant-Based Antibiotic Research Against Quorum Sensing and Biofilm Formation in Pseudomonas aeruginosa and Escherichia coli Multiresistant Microbes. Biomolecules, 16: 197.
Salsabila, A. P., Euis, J., Natsuko, K., & Dikdik, K. (2025). The Potential of Secondary Metabolites in Medicinal Plants as Anti–Quorum Sensing in Biofilms: A Comprehensive Review. Wiley Journal of Chemistry, 2025: 8838140.
Soto-Aceves, M. P.; Cocotl-Yanez, M.; Servin-Gonzalez, L.; & Soberon-Chavez, G. (2021). The Rhl Quorum-Sensing System Is at the Top of the Regulatory Hierarchy under Phosphate-Limiting Conditions in Pseudomonas aeruginosa PAO1. J. Bacteriol. 203: e00475-20. https://doi.org/10.1128/JB.00475-20
Touati, A., Ibrahim, N. A., Tighilt, L., & Idres, T. (2025). Anti-QS Strategies Against Pseudomonas aeruginosa Infections. Microorganisms, 13: 1838. https://doi.org/10.3390/microorganisms13081838
Wang, R., Wang, S., Liu, L., Qiu, C., Xiao, S., Ouyang, Q., & Ji, M. (2025). Research Progress on the Influence Factors of the Quorum Sensing System Regulating the Growth ofWastewater Treatment Biofilm. Water, 17: 1944. https://doi.org/10.3390/w17071944
Yang, D., Hao, S., Zhao, L., Shi, F., Ye, G., Zou, Y., Song, X., Li, L., Yin, Z., He, X., Feng, S., Chen, H., Zhang, Y., Gao. Y., Li, Y., & Tang, H., (2021). Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa. Front. Microbiol. 12: 692474. https://doi.org/10.3389/fmicb.2021.692474
Yehia, H. M., Salem-Bekhitn, M. M., & Mostafa, A. (2020). Bacterial identification using 16S rRNA sequencing in veterinary settings. Journal of Veterinary Diagnostics, 13: 34–42.
Citations |
 |
 |
 |
 |
