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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 15, Issue:4, April, 2026

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2026.15(4): 217-227
DOI: https://doi.org/10.20546/ijcmas.2026.1504.027


Anaerobic Co-digestion of Neem (Azadirachta indica A. Juss) Pulp with Cattle Manure: Effect of Mixing Ratio and Dry Matter Content on Biogas Production
Laboratory for Research on Agricultural Resources and Environmental Health (LARASE), Higher School of Agronomy (ESA), University of Lomé - 10 BP 1515; Lomé, Togo
*Corresponding author
Abstract:

Neem (Azadirachta indica A. Juss) pulp, a by-product of biopesticide production, constitutes a phytosanitary waste whose management poses environmental challenges. Its energy valorization through anaerobic digestion could transform this waste into a renewable resource. This study aimed to evaluate the effect of neem pulp/cattle manure co-digestion ratio and dry matter content on biogas production. Batch trials (200 L digesters) were conducted over 45 days under tropical ambient temperature (29-37°C). Four co-digestion ratios (P100: 100 % pulp; P75B25: 75 % pulp + 25 % manure; P50B50: 50 % pulp + 50 % manure; B100: 100 % manure) and two dry matter contents (TS10: 10 % dry matter; TS15: 15 % dry matter) were tested in triplicate. Results showed that pulp alone (P100) completely inhibited methanogenesis (12.2 L in 45 days, final pH 4.10-4.14) due to its bioactive compound content (azadirachtin, salanine, nimbin). Co-digestion with cattle manure progressively alleviated this inhibition through its buffering capacity and inhibitor dilution. The optimal ratio was P50B50 at TS15 (332.4 L in 45 days). A high significant interaction (p < 0.001) between co-digestion ratio and dry matter content was demonstrated. This study shows that controlled co-digestion enables energy valorization of neem based biopesticide waste under tropical climate, thus paving the way toward a circular economy in agricultural systems.


Keywords: Azadirachta indica; Anaerobic digestion; Biogas production; bioactive compounds; tropical climate


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How to cite this article:

MEMOKO Djalalou-Dine, AMENUTI Yao Félicité and NENONENE Yawo Amen. 2026. Anaerobic Co-digestion of Neem (Azadirachta indica A. Juss) Pulp with Cattle Manure: Effect of Mixing Ratio and Dry Matter Content on Biogas Production.Int.J.Curr.Microbiol.App.Sci. 15(4): 217-227. doi: https://doi.org/10.20546/ijcmas.2026.1504.027
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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