National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacterial disease of fresh water fish causes significant reduction of fish production leading to huge economic loss. This study aims to investigate and isolate bacterial pathogens from gills, kidney and intestine of the fresh water cultured fish and to study antibiotic resistance. The strains of Proteus mirablis, Aeromonas veronii, Micrococcus luteus, Staphylococcus succinus and Bacillus sp. have been isolated from the gills, kidney and intestine and which was later identified on the basis of biochemical tests and 16S rRNA gene sequencing. Antibiotic sensitivity patterns of the bacterial isolates were tested against eight commonly used antibiotics viz., ampicillin, amoxicillin, azithromycin, cefalexin, ciprofloxacin, kanamycin, streptomycin and tetracycline. Proteus mirablis showed resistance to ampicillin, amoxycillin, cefalexin, kanamycin, streptomycin and tetracycline, Aeromonas veronii and Staphylococcus succinus was highly resistant only to cefalexin and ampicillin while Bacillus sp. showed high to moderate resistance to ampicillin, amoxycillin and cefalexin. The presence of antibiotic-resistant pathogenic bacteria in cultivated fish is a very detrimental threat to human health. So, need for implementing measures that guard against the misuse of antimicrobial drugs in fish feeds is therefore emphasized.
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