National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In search of potential source of biodiesel, four microalgal strains were finally isolated. In the first step of investigation, 25 samples from aquatic bodies of various districts of north India were collected and processed, resulting in a total of 4 microalgal strains viz. Nostoc sp., Chlorella sp., Chlorococcum sp. and Oscillatoria sp. were isolated and grown in pure culture in culture room. On the basis of observations, maximum lipid content was recorded as 19.88% (on dry biomass basis) in Chlorella fw-12 (at 28th day) under standard growth conditions in liquid BG-11 medium. Extracted lipid was purified by trans-esterification and characterized by GC-MS technique in respect to lipid quality (profiling of lipid constituents). A total of 10 chemical compounds were detected as saturated hydrocarbons namely n-Decane, n-Undecane, n-Dodecane, n-Tridecane, n-Tetradecane. Other than hydrocarbons, a high percentage of fatty acids (61.44%) was also recorded, major fatty acid recorded were C19:1, C19:2 and C19:3, which were methyl hexadecanoate (palmitate) (30.20%), methyl 9, 12, 15-octadecatrienoate (linolenate) (11.03%), methyl octadecaenoate (elaidate) (8.74%), methyl 9, 12-octadecadienoate (linolenate) (7.51%) and methyl 5, 9, 12-octadecatrienoate (gamma-linolenoate) (2.07%), which makes better quality of algal biodiesel. Thus, selected Chlorella fw-12 unicellular green algae would be a potent source of qualitative biodiesel.
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