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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1801-1810
DOI: https://doi.org/10.20546/ijcmas.2017.605.196


Extraction and Profiling of Lipids of Isolated Strain Chlorella fw-12 by GC-MS
Parveen Kumar1*, Devendra Kumar2, Priyanka Nehra2 and P.K. Sharma3
1Department of Microbiology, C. C. S. University, Meerut, India
2Department of Microbiology, Kurukshetra University Kurukshetra, Haryana
3Department of Genetics and Plant Breeding, C. C. S. University, Meerut, India
*Corresponding author
Abstract:

In search of potential source of biodiesel, four microalgal strains were finally isolated. In the first step of investigation, 25 samples from aquatic bodies of various districts of north India were collected and processed, resulting in a total of 4 microalgal strains viz. Nostoc sp., Chlorella sp., Chlorococcum sp. and Oscillatoria sp. were isolated and grown in pure culture in culture room. On the basis of observations, maximum lipid content was recorded as 19.88% (on dry biomass basis) in Chlorella fw-12 (at 28th day) under standard growth conditions in liquid BG-11 medium. Extracted lipid was purified by trans-esterification and characterized by GC-MS technique in respect to lipid quality (profiling of lipid constituents). A total of 10 chemical compounds were detected as saturated hydrocarbons namely n-Decane, n-Undecane, n-Dodecane, n-Tridecane, n-Tetradecane. Other than hydrocarbons, a high percentage of fatty acids (61.44%) was also recorded, major fatty acid recorded were C19:1, C19:2 and C19:3, which were methyl hexadecanoate (palmitate) (30.20%), methyl 9, 12, 15-octadecatrienoate (linolenate) (11.03%), methyl octadecaenoate (elaidate) (8.74%), methyl 9, 12-octadecadienoate (linolenate) (7.51%) and methyl 5, 9, 12-octadecatrienoate (gamma-linolenoate) (2.07%), which makes better quality of algal biodiesel. Thus, selected Chlorella fw-12 unicellular green algae would be a potent source of qualitative biodiesel.


Keywords: Algae culture, Microalgal lipids, Transesterification, GC-MS, biodiesel.
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How to cite this article:

Parveen Kumar, Devendra Kumar, Priyanka Nehra, P.K. Sharma. 2017. Extraction and Profiling of Lipids of Isolated Strain Chlorella fw-12 by GC-MSInt.J.Curr.Microbiol.App.Sci. 6(5): 1801-1810. doi: https://doi.org/10.20546/ijcmas.2017.605.196