National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The laboratory studies were conducted at Division of Plant Pathology and Agricultural Microbiology, Rajarshee Chhatrapati Shahu Maharaj College of Agriculture, Kolhapur for the isolation, purification and characterization of isolates obtained from rhizosphere soil of Marigold fields completely randomized design (CRD) was used for laboratory studies. Ten isolates were obtained from fifteen soil samples collected from rhizosphere soil of marigold fields from different locations of Kolhapur district. They were designated as ATSS1, ATSN2, ATSA3, ATHM1, ATHH2, ATHA3, ATHN4, ATKK1, and ATKB2 and ATKM3. All the isolates were studied for the morphological, cultural and biochemical characterization. In the morphological studies all the isolates found Gram negative and stained pink in color, positive in motility and also showed positive results to KOH test. All of these isolates showed the white creamy to white color, out of 10 isolates 8 circular were (cocci) shaped with irregular wavy margin and flat elevation and 2 were rod shaped. All the isolates were tested for biochemical tests namely starch hydrolysis, gelatin hydrolysis, gas production, H2S production, oxidase, methyl red test and catalase test. All of them showed the positive results to all the test except ATHH2 which gave negative results to gelatin hydrolase and starch hydrolase. These isolates were cultured on five different medias for their cultural characterization which has resulted that the isolates showed superior growth on Ashby’s media and isolates ATHM1 and ATKM3 showed superior growth with colony diameter of 2.50cm and 2.27cm respectively. Nitrogen fixation in N free broth isolate ATHM1 significantly showed highest nitrogen fixation (18.68mg/ml) followed by ATKM3(15.60mg/ml) and selected as efficient isolates. Out of them two isolates (ATHM1 and ATKM3) were found superior over other isolates which were collected from Male village, Hatkanagle tehsil and Mudshingi village, Karvir tehsil of Kolhapur district respectively.
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