National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Foodborne illnesses remain a critical public health challenge in sub-Saharan Africa. In Burkina Faso, raw meat and offal are staple protein sources, yet data regarding their microbiological safety and the genetic virulence of circulating pathogens remain limited. This study aimed to determine the prevalence of Salmonella enterica in raw beef, mutton, and intestines sold in Ouagadougou markets and to characterize the distribution of key virulence genes among the isolates. A total of 450 samples, including raw beef (n=175), mutton (n=175), beef intestines (n=50), and mutton intestines (n=50), were collected from 25 local markets. Salmonella isolation and identification were performed according to ISO 6579-1:2017 standards and API 20E galleries. Molecular characterization of virulence markers (invA, fimA, stn, spvR, and spvC) was conducted using PCR. The overall prevalence of Salmonella was 19% (86/450). Contamination rates were highest in raw beef (27%) and mutton intestines (22%), followed by beef intestines (18%) and raw mutton (11%). Molecular analysis revealed that fimA was the most frequent gene (89.53%), followed by stn and invA (76.74%). Notably, the plasmid-borne systemic virulence genes spvC and spvR were detected in 55.81% and 41.86% of isolates, respectively. Remarkably, 100% of the isolates recovered from intestines carried all five investigated virulence genes. The high prevalence of multidimensional virulence profiles, particularly in offal, poses a severe risk of systemic infections for consumers. These findings underscore the urgent need for improved slaughtering hygiene and the modernization of retail infrastructures, such as the use of elevated stalls and single-use packaging, to ensure food safety in Burkina Faso.
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