National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pigeon pea (Cajanus cajan L.) is a legume with an important source of proteins, grown in several tropical and subtropical regions. However, despite its great importance, especially in tropical regions facing food and nutritional insecurity, pigeon pea remains one of the oldest and least valued food crops in West Africa, particularly in Senegal. The aim of this study was to contribute to a better understanding of the diversity of rhizobia associated with three pigeon pea provenances in three regions of Senegal (Fatick, Nioro and Kaffrine), with a view to their exploitation as microbial biofertilizers. Soil samples were collected from rhizosphere of pigeon pea plants in the three regions. Shadehouse trials were carried out to determine the most probable number of bacteria (MPN), as well as the phenotypic and genetic diversity of rhizobia by trapping with pigeon pea seeds from the three provenances. The results revealed a variability in MPN between the three study sites. Bacterial isolation from root nodules determined that pigeon pea associates more with fast-growing bacteria. A collection of 87 isolates was obtained from the plant nodules cultivated in the three sites. Amplification of the rDNA ITS region, followed by enzymatic digestion, disclosed 35 genetic profiles, including 4 common to Fatick and Kaffrine, 2 to Fatick and Kaffrine, 1 to Nioro and Kaffrine, 8 specific to Fatick, 8 to Kaffrine and 5 to Nioro. At the end of the infectivity test with 56 bacterial isolates, considered under controlled conditions, only 29 re-infected the host-plant. A positive effect of inoculation with some bacterial isolates (F12p, N23, N22, N5, K3 and K16p) was noted for chlorophyll content and certain plant growth parameters (nodule number and weight, shoot and root dry weight). This study underlined that some of the bacterial strains may be potential candidates for improving growth and productivity of pigeon pea in Senegal.
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