National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Enterobacteriaceae are frequently implicated in urinary tract infections and pose a significant public health concern. The objective of this study was to detect beta-lactam resistance genes in Enterobacteriaceae communities isolated from the urine of patients with urinary catheters. 27 bacterial strains were collected and identified using standard bacteriological and biochemical tests. Phenotypic characterization to antibiotics was determined by the antibiotic disk diffusion method. The production of extended-spectrum beta-lactamases was determined by the double synergy method. Finally, the detection of bla genes was performed by polymerase chain reaction. Of the collected Enterobacteriaceae, 37% came from the outpatient department and 29.69% from the Nephrology department (χ² = 6.6000; p = 0.472). Regarding bacterial resistance to antibiotics, the results indicate that isolates treated with imipenem were highly susceptible (85.19%). However, Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae exhibited resistance rates ranging from 45% to 80% against third-generation cephalosporins. Of the 27 strains, 14 produced extended-spectrum beta-lactamases (44.26%). Genotyping revealed that the blaSHV gene was detected in 13 out of 20 strains (65%). The blaTEM gene was detected in 14 out of 20 strains (70%), and the blaCTX-M1 gene in 11 out of 20 strains (55%). This study highlights the involvement of Enterobacteriaceae harboring beta-lactam resistance genes. Faced with this situation, it is wise to pay particular attention to curbing the spread of such strains.
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