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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1753-1758
DOI: https://doi.org/10.20546/ijcmas.2017.605.190


Relative Expression of TLR9 Gene in Natural Sub-clinical and Clinical Cases of Bovine Mastitis caused by Escherichia coli
R. Lakshmi1*and K.K. Jayavardhanan2
1Department of Veterinary Biochemistry, Rajiv Gandhi Institute of Veterinary
Education and Research, Puducherry - 605 009, India
2Department of Veterinary Biochemistry, College of Veterinary and Animal Sciences,Thrissur, Kerala – 680651, India
*Corresponding author
Abstract:

Mastitis is an inflammatory condition of the mammary tissue resulting in several physiological and metabolic changes, trauma, and more frequently it is caused by contagious or environmental pathogenic microorganisms, including Gram-positive and Gram-negative bacteria. Escherichia coli are most common infectious agents responsible for sub-clinical and clinical mastitis in dairy forms. The first line of defense against mammary bacterial infection was initiated by innate resistance of mammary gland. Toll-like receptors (TLRs) are a key family of innate immune proteins, serving as the principal recognition site of pathogens and signalling functions, enabling the host to eliminate pathogens. Hence, this study assessed the expression of an important TLR9 gene in spontaneous bovine sub-clinical and clinical mastitis caused by E. coli. The total RNA was isolated from milk somatic cells converted as cDNA using oligo (dT) primers. Relative quantitation of mRNA of TLR9 was analysed by RT-qPCR system. The mRNA expression of TLR9 gene in sub-clinical mastitis was higher (3.22 fold) than clinical case (1.09 fold) when compared to normal bovine case. Relative difference in the expression of TLR9 gene in sub-clinical and clinical mastitis was found to be significant (P ≤ 0.05). During sub-clinical stage of infection the expression of TLR9 gene was at high level, therefore most of the sub-clinical mastitis subsided by itself without precipitating into clinical mastitis. TLR9 mediated signalling cascades will be fundamental to understand the host immune response against bovine mastitis.


Keywords: TLR9, Expression, Mastitis and E. coli
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How to cite this article:

Lakshmi, R. and Jayavardhanan, K.K. 2017. Relative Expression of TLR9 Gene in Natural Sub-clinical and Clinical Cases of Bovine Mastitis caused by Escherichia coliInt.J.Curr.Microbiol.App.Sci. 6(5): 1753-1758. doi: https://doi.org/10.20546/ijcmas.2017.605.190