Evaluation of Disinfectants by Surface Challenge test as per USP 1072 standard at Pharma Company Laboratory, India
Infiniti Lines, Mumbai, India
*Corresponding author
Abstract:
Cleaning and disinfection of surfaces are essential steps for maintaining the cleanliness of pharmaceutical manufacturing operations. To validate the efficacy of disinfectants used in disinfection procedure, in order to reduce surface contaminations, we tested invitro the action of commercial disinfectants. Surface Challenge testing has been carried in a Pharmaceutical manufacturing laboratory in accordance with the United States Pharmacopeia <1072> Disinfectants and Antiseptics protocol. Surface Challenge tests assess the effectiveness of the selected disinfectant against surface-adhered microorganisms. The test organisms used include standard strains mentioned in USP <1072>. Our results showed a bactericidal, fungicidal, and sporicidal efficacy coherent to the acceptance criteria suggested by United States Pharmacopeia <1072>. The correct implementation of our cleaning and disinfection procedure, respecting stipulated concentrations and contact times, led to a reduction of more than 4 Log10 for all microorganisms used. The qualification of procedure was carried out on a screening test in order to measure effectiveness of the disinfectants, chosen according to their principle of action.
Keywords: Disinfection, , United States Pharmacopeia <1072>, qualification, Log10 reduction, contamination
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How to cite this article:
Imran Memon, Naushin I. Memon, Surjeet Samanta and Rahul Mali. 2025. Evaluation of Disinfectants by Surface Challenge test as per USP 1072 standard at Pharma Company Laboratory, India.
Int.J.Curr.Microbiol.App.Sci. 14(11): 195-210. doi:
https://doi.org/10.20546/ijcmas.2025.1411.020
Citations
National Academy of Agricultural Sciences (NAAS) 
