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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 14, Issue:11, November, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(11): 169-176
DOI: https://doi.org/10.20546/ijcmas.2025.1411.017


Chromatographic Analysis of Ethyl Acetate Extract of Endophytic Bacteria Isolated from Paederia foetida
Sonali Priyadarshini and Chandi Charan Rath*
Department of Life Sciences, Rama Devi Women’s University, Vidya Vihar, Bhubaneswar - 751022, Odisha, India
*Corresponding author
Abstract:

In the present study ten endophytic bacteria were isolated from Paederia foetida and were subjected to antibacterial activity test. The maximum zone of inhibition was observed by the isolate PFNP2 against Staphylococcus aureus (17.6±0.5 mm) followed by Salmonella sp. (15.3±0.5 mm) and MRSA (14.6±0.5 mm). The bacterial isolate was identified as Staphylococcus haemolyticus by 16s rRNA gene sequencing with NCBI accession number PQ143273. Genes that are essential for the adaptation to the plants were mainly acquired by horizontal gene transfer which could explain the adaptation of such bacteria to plant environment. Presence of aromatic, aliphatic (C-H) and carboxylic acid (C=O) groups were detected in the ethyl acetate extract of the selected isolate by FTIR analysis. Bioactive compounds such as 2- Piperidinone, N- Hexadecanoic acid and Pyrrolo [1,2-a] pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)- identified from the GC-MS mass spectra of the bacterial extract have been reported to have antimicrobial properties. This indicates that bacterial endophytes from Paederia foetida could be a source of antimicrobial compounds for use in biotechnological and pharmaceutical applications.


Keywords: Endophyte, Paederia foetida, BLIS, FTIR, GC-MS


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How to cite this article:

Sonali Priyadarshini and Chandi Charan Rath. 2025. Chromatographic Analysis of Ethyl Acetate Extract of Endophytic Bacteria Isolated from Paederia foetida.Int.J.Curr.Microbiol.App.Sci. 14(11): 169-176. doi: https://doi.org/10.20546/ijcmas.2025.1411.017
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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