National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Aspergillus aflatoxiformans was isolated from the rhizosphere soil of Madhubani, Bihar, India, and identified through ITS sequencing and phylogenetic analysis (GenBank accession number: PV789347). Antagonistic activity against Mucor irregularis (GenBank accession no. PV789341) was evaluated on potato dextrose agar (PDA) using the dual culture technique, which showed a mean percentage inhibition of radial growth (PIRG) of 80. 9 ± 4. 08%. Chitinase activity was quantified by measuring the hydrolysis zone diameter in six replicate plates, with a mean value of 2. 99 ± 0. 21 cm and a coefficient of variation of 7. 12%. Protease activity showed a mean proteolytic zone diameter of 3. 34 ± 0. 37 cm (n = 9). For plant interaction assessment, a 5-day-old broth culture containing approximately 1 × 10? spores mL?¹ was prepared and 10 ml volume applied as a foliar and root spray to Tagetes erecta (marigold) every 15 days during evening time. No significant differences were observed in treated plants compared to controls, indicating the absence of phytotoxic effects. These findings highlight A. aflatoxiformis from Madhubani as a potentially safe and enzymatically active antagonist against plant pathogens, supporting its further exploration as a biocontrol agent in sustainable agriculture.
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