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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 14, Issue:11, November, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(11): 27-35
DOI: https://doi.org/10.20546/ijcmas.2025.1411.004


Isolation and Characterization of Lacticaseibacillus paracasei with Nicotine-Degrading and Catechu Stain-Reducing Potential
Bhavika Chauhan and Nileshkumar Pandya
Department of Microbiology, Arts, Science & Commerce College, Kamrej Cross Road, Surat, Gujarat-394185, India (Affiliated to Veer Narmad South Gujarat University, Surat)
*Corresponding author
Abstract:

The increasing consumption of gutkha, a smokeless tobacco product, poses severe public health risks due to its high nicotine, areca nut alkaloids, slaked lime and catechu—substances known to cause serious oral and systemic health problems. Nicotine is the major addictive and toxic compound, while catechu and lime contribute to severe staining of oral tissues and public spaces. This study investigates the probiotic bacterium Lacticaseibacillus paracasei for its potential to degrade nicotine and reduce catechu-induced stains. L. paracasei was isolated from fermented milk products and identified based on morphological and molecular characteristics. Nicotine degradation and stain-reducing activities were quantified spectrophotometrically. The Lacticaseibacillus paracasei isolate exhibited significant nicotine degradation efficiency and noticeable reduction of catechu stains. These findings suggest that L. paracasei can serve as a potential bio-agent for developing probiotic formulations or modified gutkha products aimed at reducing tobacco-related toxicity and improving public hygiene.


Keywords: Probiotic bacteria, Gutkha, Nicotine degradation, Stain reduction,;Lacticaseibacillus paracasei, Public health


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How to cite this article:

Bhavika Chauhan and Nileshkumar Pandya. 2025. Isolation and Characterization of Lacticaseibacillus paracasei with Nicotine-Degrading and Catechu Stain-Reducing Potential.Int.J.Curr.Microbiol.App.Sci. 14(11): 27-35. doi: https://doi.org/10.20546/ijcmas.2025.1411.004
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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