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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2023: 95.56
Index Copernicus ICI Journals Master List 2023 - IJCMAS--ICV 2023: 95.56
For more details click here
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Original Research Articles                      Volume : 14, Issue:10, October, 2025
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
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Int.J.Curr.Microbiol.App.Sci.2025.14(10): 106-125
DOI: https://doi.org/10.20546/ijcmas.2025.1410.010


Bacteriological Profile of Non-Fermenting Gram Negative Bacilli and its Antibiogram in Tertiary Care Hospital, Calicut, India
Gayathri Meyana1*, Reshmi Gopalakrishnan2, M. Savitha3, Swathy Viswanath1, M. P. Anjali1, M. M. Athulya1, Irfana1 and P. P. Vismaya1
1Department of Microbiology, MIMS college of Allied Health Sciences, Kerala University of Health Sciences, Malappuram, India 2Department of Microbiology, Aster MIMS Hospital, Calicut, India 3Department of Microbiology, MIMS college of Allied Health Sciences, Malappuram, India 4Department of Microbiology, MIMS college of Allied Health Sciences, Malappuram, India
*Corresponding author
Abstract:

Non-fermenting Gram-negative bacilli (NFGNB) have emerged as a major cause of healthcare-associated infections and are innately resistant to many antibiotics. High intrinsic resistance of NFGNB to antimicrobial compounds makes the treatment caused by them difficult and expensive. To identify and determine the antibiogram of Nonfermenting gram negative bacilli isolated in clinical samples and to compare the its pattern over a period of 2 years. This ambidirectional study was done at our Department of Microbiology Aster MIMS, Calicut. NFGNB were isolated from a variety of clinical specimens, plated on blood agar and MacConkey agar and incubated at 37°C for 18–24 h under aerobic conditions. Appropriate biochemical tests were done to identify the organisms isolated. Antibiotic susceptibility test was performed using the modified Kirby–Bauer disc diffusion method using commercially available discs on Mueller–Hinton agar. Blood culture was done by automated blood culture system, (BacT/Alert 3 D) and identification and antibiotic susceptibility of non-fermenting gram-negative bacilli was done by VITEK 2 Compact System. Out of 28162 clinical samples, 2148 (1109 in year 2023 and 1038 in year 2024) (7.6%) yielded NFGNB. Acinetobacter baumanni was the most common NFGNB, isolated in 965/2148 (44.9%) samples which were susceptibile to colistin (100%) and tigecycline (66.9%) , followed by Pseudomonas aeruginosa in 700/2148 (32.5%) susceptible to colistin (98.2%) and amikacin (75.7%) and a total of 965/2148 (44.9%) carbapenem-resistant Acinetobacter baumannii (CRAB) and 700/2148 (32.5%) carbapenem-resistant Pseudomonas aeruginosa (CRPA) was detected in the study. This study revealed a significantly high prevalence of non-fermenting Gram-negative bacilli (NFGNB), with Acinetobacter baumannii and Pseudomonas aeruginosa being the most frequently isolated species. The high rates of. multidrug-resistant A. baumannii and P. aeruginosa underscore a growing concern regarding the rapid emergence and spread of antimicrobial resistance in the region. These findings reflect the rising threat of multidrug resistance among NFGNB and emphasize the urgent need for enhanced antimicrobial stewardship, infection control, and routine resistance surveillance to contain their spread and preserve treatment efficacy.


Keywords: Non-fermenting Gram-negative bacilli, Antibiotic susceptibility, carbapenem-resistant

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How to cite this article:

Gayathri Meyana, Reshmi Gopalakrishnan, Savitha M., Swathy Viswanath, Anjali M. P., Athulya M. M., Irfana and Vismaya P. P. 2025. Bacteriological Profile of Non-Fermenting Gram Negative Bacilli and Its Antibiogram in Tertiary Care Hospital, Calicut.Int.J.Curr.Microbiol.App.Sci. 14(10): 106-125. doi: https://doi.org/10.20546/ijcmas.2025.1410.010
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