Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2023 - IJCMAS--ICV 2023: 95.56 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer


See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 14, Issue:9, September, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(9): 58-67
DOI: https://doi.org/10.20546/ijcmas.2025.1409.007


Studies on Microbial Fermentation and Production of Yogurt
P. Elamparithi1*, A. Harini2, S. Kartheeswari2, V. Mukesh Kumar2, S. Poojegan2, T. Priyadharshini2, M. Rexcy Blesslin2, K. Sivasakthi2, A. Sowbaraniga2, M. Subalakshmi2 and S. Sujitha2

1Department of Agricultural Microbiology, Imayam Institute of Agriculture and Technology, Thuraiyur, India
2Imayam Institute of Agriculture and Technology, Thuraiyur, India


*Corresponding author
Abstract:

This study was carried out to investigate microbial fermentation and yogurt production with special emphasis on lactic acid bacteria (LAB) and vegetable juice fortification. Yogurt samples were collected from different sources and analyzed for total microbial load using standard plate count methods. LAB were isolated and purified on MRS agar, and characterized using cultural, morphological, and biochemical techniques. Further, carrot and beetroot juices at varying concentrations (0.1%, 1%, 2%, and 5%) were incorporated into yogurt to evaluate their effect on physicochemical and sensory properties. Results showed that both Vijay curd (C1)(2.6 × 10? cfu/mL) and Hostel curd (C2)(5.3 × 10? cfu/mL) contained abundant LAB populations. Carrot juice fortification caused a gradual decrease in pH from 6.0 to 3.0, with increasing firmness, pale orange coloration, and stronger acidic odour. Beetroot juice addition led to a more pronounced drop in pH (1–3), accompanied by creamy to reddish-pink coloration and stronger flavour development. In both treatments, shelf life remained constant at 3 days, and replication trials showed reproducibility of results. Overall, 20% juice fortification was found to provide the best balance between flavour, acidity, colour, and texture. Between the two replications, C1 performed better than C2 in both carrot and beetroot yogurt samples, showing stronger flavour, lower pH, and improved overall fermentation quality. The findings highlight the potential of using LAB fermentation along with natural vegetable fortification to develop functional yogurts with enhanced nutritional and sensory attributes.


Keywords: Yogurt, Lactic Acid Bacteria, Carrot and Beetroot Juices


References:

Adams, M. R., & Moss, M. O. (2000). Food microbiology (2nd ed.). Royal Society of Chemistry.

Adjei, M. L., Boakye, A. A., Deku, G., Pepra-Ameyaw, N. B., Simpah Anim Jnr, A., Oduro, I. N., & Ellis, W. O. (2024). Development of yoghurt incorporated with beetroot puree and its effect on the physicochemical properties and consumer acceptance. Heliyon, 10(3), e25492. https://doi.org/10.1016/j.heliyon.2024.e25492

Agagunduz, D., Urun, Y., & Demirkol, A. (2021). Probiotic characteristics and functional benefits of lactic acid bacteria in dairy products. Food Science and Nutrition, 9(12), 6626–6635.

Aguirre-Garcia, Y. L., Cerda-Alvarez, N. C., Santiago-Santiago, R. M., Chantre-López, A. R., Rangel-Ortega, S. D. C., & Rodríguez-Herrera, R. (2025). The metabolites produced by lactic acid bacteria and their role in the microbiota–gut–brain axis. Fermentation, 11(7), 378. https://doi.org/10.3390/fermentation11070378    

Aktas, B., Demirkol, A., & Yildiz, O. (2024). Technological and probiotic traits of lactic acid bacteria: Advances and applications. Journal of Dairy Science, 107(1), 125–136.

Aly, S. A., Galal, E. A., & Elewa, N. A. (2004). Carrot yoghurt: Sensory, chemical, microbiological properties and consumer acceptance. Pakistan Journal of Nutrition, 3(6), 322–330. https://doi.org/10.3923/pjn.2004.322.330

Aygun, A., & Durmaz, Y. (2017). Development of probiotic yogurt containing potato juice. Food Science and Technology, 37(1), 66–72.

Çetin, B., Kaya, A., & Aksu, H. (2025). Isolation and screening of lactic acid bacteria from traditional yogurts for functional dairy product development. Journal of Applied Microbiology, 138(3), 311–324.

El Ahmadi, B., Lahlali, R., & Zair, T. (2025). Isolation and preliminary screening of lactic acid bacteria with antimicrobial properties from raw milk. Probiotics and Antimicrobial Proteins, 17(2), 98–105. https://doi.org/10.3389/fmicb.2025.1565016

 El-Baradei, G., Delacroix-Buchet, A., & Ogier, J. C. (2022). Bacterial biodiversity of traditional fermented dairy products. Food Microbiology, 103, 104-110.

FDA. (2020). Code of Federal Regulations: Title 21, Part 131—Milk and cream. U.S. Food and Drug Administration.

Fernandes, L., Silva, A. R., & Costa, J. (2022). The influence of pH on volatile compound production in yogurt. Dairy, 3(1), 134–145.

Hank Thi Hong Nguyen, H., Ong, L., Lefèvre, C., Kentish, S., & Gras, S. (2014). The impact of milk composition on yogurt properties. International Dairy Journal, 43, 1–7.

Janiszewska-Turak, E., Pobiega, K., Rybak, K., Synowiec, A., Wo?niak, ?., Trych, U., Gniewosz, M., & Witrowa-Rajchert, D. (2023). Changes in physical and chemical parameters of beetroot and carrot juices obtained by lactic fermentation. Applied Sciences, 13(10), 6113. https://doi.org/10.3390/app13106113

Kim, D., & Cho, Y. (2024). Storage stability and textural changes in yogurt under varying pH conditions. Journal of Dairy Technology, 76(4), 256–263.

Kumar, P., Singh, S., & Yadav, B. (2023). Physicochemical parameters affecting yogurt quality. Journal of Food Biochemistry, 47(3), e13576.

Liu, Y., Wang, H., & Zhao, L. (2024). Improving antioxidant properties of goat milk yogurt with Limosilactobacillusfermentum. Dairy Science & Technology, 104(2), 89–101.

Mangiapane, E., Viti, C., & Puglisi, E. (2015). Lactic acid bacteria: Diversity and ecology in dairy environments. Annals of Microbiology, 65, 1–11.

Nahar, A., Sultana, S., & Begum, R. (2007). Study on the quality attributes of yogurt prepared from cow, goat, and buffalo milk. Pakistan Journal of Nutrition, 6(6), 607–611.  https://doi.org/10.3923/pjn.2007.607.611

Olatidoye, O. P., Sobowale, S. S., & Akinlotan, J. V. (2017). Yogurt production and its microbial significance. Journal of Food Safety and Hygiene, 3(2), 67–75.

Ramesh, P., Kumar, A., & Sharma, M. (2024). Relationship between pH and gel strength in yogurt. International Dairy Journal, 139, 105493.

Rashed Rashid Anjum, M., Zahoor, T., & Latif, M. (2007). Functional dairy products and their health benefits. Pakistan Journal of Nutrition, 6(4), 377–381.

Singh, R., & Patel, M. (2023). The impact of pH and fermentation duration on yogurt texture and mouthfeel. Food Structure, 37, 100296.

Tamime, A. Y., & Robinson, R. K. (2007). Yoghurt: Science and technology (3rd ed.). Woodhead Publishing.

Tewari, A., Bhatt, D. K., & Negi, P. (2019). Probiotic properties of traditional fermented dairy products. Journal of Food Research and Technology, 7(2), 23–30.

Yang, S., Yan, D., Zou, Y., Mu, D., Li, X., Shi, H., Luo, X., Yang, M., Yue, X., Wu, R., & Wu, J. (2021). Fermentation temperature affects yogurt quality: A metabolomics study. Food Bioscience, 43, Article 101104. https://doi.org/10.1016/j.fbio.2021.101104

Zhang, Y., Wu, C., & Feng, X. (2024). Influence of fermentation temperature on yogurt quality. Journal of Dairy Research, 91(2), 111–120.

Download this article as Download

How to cite this article:

Elamparithi, P., A. Harini, S. Kartheeswari, V. Mukesh Kumar, S. Poojegan, T. Priyadharshini, M. Rexcy Blesslin, K. Sivasakthi, A. Sowbaraniga, M. Subalakshmi and Sujitha, S. 2025. Studies on Microbial Fermentation and Production of Yogurt.Int.J.Curr.Microbiol.App.Sci. 14(9): 58-67. doi: https://doi.org/10.20546/ijcmas.2025.1409.007
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

Citations