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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 14, Issue:9, September, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(9): 8-15
DOI: https://doi.org/10.20546/ijcmas.2025.1409.002


Probiotic Potential and Safety Assessment of Lactic Acid Bacteria from Naturally Fermented Milk in Karnataka: Molecular Characterization and Functional Traits
1Department of Microbiology, Davangere University, Davangere, Karnataka, India
2Department of Biomedical Science, SDM Research Institute for Biomedical Sciences, Shri Dharmasthala Manjunatheshwara University, Dharwad, Karnataka, India

*Corresponding author
Abstract:

The strains of the lactic acid bacteria that were identified in Karnataka, from naturally fermented milk, are the subject of this investigation to assess their probiotic potential and safety. Ten representative strains of LAB were identified through sequencing of 16S rRNA genes, includes Lactiplantibacillus plantarum, Lactobacillus fermentum, Ligilactobacillus murinus, Leuconostoc mesenteroides, Enterococcus faecalis, Lactiplantibacillus plantarum, Lacticaseibacillus rhamnosus, Enterococcus lactis, Lactobacillus sakei, and Lactobacillus paracasei.. Functional characterization revealed that nine strains exhibited β-galactosidase activity, beneficial for lactose digestion, and all strains produced exopolysaccharides, which improve gut health and product texture. Hemolysis testing confirmed non-hemolytic in all strains, ensuring their safety. The study highlights the probiotic promise of these LAB strains for enhancing dairy products, particularly for lactose-intolerant individuals, and underscores the safety of most isolates for probiotic applications. Further research will focus on optimizing these strains for functional roles in human health, positioning traditional fermented milk as a valuable source of natural probiotics.


Keywords: Lactic acid bacteria, Probiotics, Fermented milk, β-galactosidase, Exo-polysaccharides, Hemolysis


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How to cite this article:

Bhavya, D. C., Ajay Kumar Oli and Virupakshaiah, D. B. M. 2025. Probiotic Potential and Safety Assessment of Lactic Acid Bacteria from Naturally Fermented Milk in Karnataka: Molecular Characterization and Functional Traits.Int.J.Curr.Microbiol.App.Sci. 14(9): 8-15. doi: https://doi.org/10.20546/ijcmas.2025.1409.002
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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