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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The ornamental fish trade has experienced rapid global growth, with goldfish (Carassius auratus) being one of the most widely traded species. However, high-density stocking, poor water quality, and transportation stress often predispose ornamental fishes to various infectious diseases, threatening their health and economic value. In this study, 65 naturally infected goldfish were collected from multiple aquarium shops in Aurangabad, Maharashtra, and examined using a multi-tiered diagnostic protocol. Parasitological investigations included wet mount examinations of gills, skin scrapings, and blood smears, which revealed the presence of common ectoparasites and Endoparasites. Bacteriological studies, including Gram staining, acid-fast staining, and biochemical assays (oxidase, catalase, TSI, citrate, motility, and indole tests), led to the identification of multiple fish-pathogenic bacteria. Fungal pathogens, primarily Saprolegnia spp., were identified based on characteristic cottony mycelial growth on infected skin and fins and confirmed using lactophenol cotton blue staining. Histopathological analysis of gill, liver, kidney, and skin tissues revealed cellular degeneration, hyperplasia, necrosis, and inflammatory infiltrates, which correlated with the severity of the infection. The integrated diagnostic approach employed in this study highlights the importance of early detection and accurate identification of pathogens in improving disease management strategies for ornamental fish culture. These findings provide a critical reference for developing prophylactic and therapeutic interventions in the Indian ornamental fish sector.
Abd Elgwad, R. A., Mahdi, M. A., Arafa, W. M., & Mahmoud, M. A. (n.d.). Pathological studies on external and internal parasitic affections of goldfish (Carassius auratus) [Unpublished manuscript]. ResearchGate.
Austin, B. (2012). Bacteria and fungi from fish and other aquatic animals: A practical identification manual (2nd ed.). CABI. https://doi.org/10.1079/9781845938055.0000
Austin, B., & Austin, D. A. (2016). Bacterial fish pathogens: Disease of farmed and wild fish (6th ed.). Springer. https://doi.org/10.1007/978-3-319-32674-0
Au-Yeung, C., Tsui, Y. L., Choi, M. H., Chan, K. W., Wong, S. N., Ling, Y. K., et al., (2025). Antibiotic abuse in ornamental fish: An overlooked reservoir for antibiotic resistance. Microorganisms, 13(4), 937. https://doi.org/10.3390/microorganisms13040937
Bergey’s Manual Trust. (n.d.). Publications. Retrieved April 17, 2025,
Declercq, A. M., Haesebrouck, F., Van den Broeck, W., Bossier, P., & Decostere, A. (2013). Columnaris disease in fish: A review with emphasis on bacterium–host interactions. Veterinary Research, 44(1), 27. https://doi.org/10.1186/1297-9716-44-27
Ferguson, H. W. (2015). Systemic pathology of fish: A text and atlas of normal tissues in teleosts and their responses in disease (2nd ed.). ESVP.
Florindo, M. C., Jerônimo, G. T., Steckert, L. D., Acchile, M., Gonçalves, E. L. T., Cardoso, L., et al., (2017). Protozoan parasites of freshwater ornamental fish. Latin American Journal of Aquatic Research, 45(5), 948–956. https://doi.org/10.3856/vol45-issue5-fulltext-10
Geetha, P. P., Dharmaratnam, A., & Swaminathan, T. R. (2022). A peek into mass mortality caused by antimicrobial resistant Edwardsiella tarda in goldfish (Carassius auratus) in Kerala. Biologia, 77(4), 1099–1106. https://doi.org/10.1007/s11756-022-01007-9
Gökp?nar, S., Akku?, G. N., & Akdeniz, S. (2023). A parasitological examination on aquarium fish sold in pet shops in K?r?kkale. Turk Parazitoloji Dergisi, 47(3), 160–165. https://doi.org/10.4274/tpd.galenos.2023.01769
Hoffman, G. L. (2011). Parasitic nematodes of freshwater fishes of Europe. ACADEMIA.
Iqbal, Z., et al., (2014). Parasitic infections of some freshwater ornamental fishes imported in Pakistan [PDF].
Lafferty, K. D., Bush, A. O., Lotz, J. M., & Shostak, A. W. (1997). Parasitology meets ecology on its own terms: Margolis et al., revisited. Journal of Parasitology, 83(4), 575–583. https://doi.org/10.2307/3284227
MacFaddin, J. F. (2000). Biochemical tests for identification of medical bacteria (3rd ed.). Lippincott Williams & Wilkins.
Mandrioli, L., Codotto, V., D’Annunzio, G., Volpe, E., Errani, F., Eishi, Y., et al., (2022). Pathological and tissue-based molecular investigation of granulomas in cichlids reared as ornamental fish. Animals, 12(11), 1366. https://doi.org/10.3390/ani12111366
Martins, M. L., Cardoso, L., Marchiori, N., & Benites de Pádua, S. (2015). Protozoan infections in farmed fish from Brazil: Diagnosis and pathogenesis. Revista Brasileira de Parasitologia Veterinária, 24(1), 1–20. https://doi.org/10.1590/S1984-29612015001
Moravec, F., & Justine, J. L. (2019). New species and new records of camallanid nematodes (Nematoda, Camallanidae) from marine fishes and sea snakes in New Caledonia. Parasite, 26, 66. https://doi.org/10.1051/parasite/2019064
Moustafa, M. M. A. E. K., et al., (2015). Saprolegniosis in goldfish (Carassius auratus), associated with Saprolegnia parasitica; molecular characterization and electron microscopy.
Mustafa, R. A., Rather, S. A., Kousar, R., Ashraf, M. V., Shah, A. A., Ahmad, S., & Khan, M. A. H. (2024). Comprehensive review on parasitic infections reported in the common fish found in UT of Jammu and Kashmir, India. Journal of Parasitic Diseases, 48(4), 736–761. https://doi.org/10.1007/s12639-024-01697-9
Pazooki, J., Masoumian, M., Yahyazadeh, M., & Abbasi, J. (2014). Survey of parasitic fauna of different ornamental freshwater fish species in Iran. Veterinary Research Forum, 5(3), 235–239. Retrieved July 17, 2025, from https://pubmed.ncbi.nlm.nih.gov/25992255/
Putt Lal, B., et al., (2024). Aquatic animal disease surveillance and important disease affecting finfish and shellfish in India. In Aquatic animal health (pp. 211–230). Springer. https://doi.org/10.1007/978-981-97-5250-8_11
Rahmati-Holasoo, R., et al., (2024). Investigating contamination with external and internal parasites in ornamental freshwater angelfish (Pterophyllum scalare). International Journal of Veterinary Research, 4(2), 44–48.
Semwal, A., Kumar, A., & Kumar, N. (2023). A review on pathogenicity of Aeromonas hydrophila and their mitigation through medicinal herbs in aquaculture. Heliyon, 9(3), e14088. https://doi.org/10.1016/j.heliyon.2023.e14088
Singh, S., Kumar, A., & Sharma, A. (2022). Mortalities in cultured Pangasianodon hypophthalmus due to oomycete Saprolegnia parasitica infection in Uttar Pradesh, India. Aquaculture Reports, 26, 101312. https://doi.org/10.1016/j.aqrep.2022.101312
Thrusfield, M. (2018). Veterinary epidemiology (4th ed.). Wiley. https://doi.org/10.1002/9781118280249
van West, P. (2006). Saprolegnia parasitica, an oomycete pathogen with a fishy appetite: New challenges for an old problem. Mycologist, 20(3), 99–104. https://doi.org/10.1016/j.mycol.2006.06.004
Whittington, R. J., & Chong, R. (2007). Global trade in ornamental fish from an Australian perspective: The case for revised import risk analysis and management strategies. Preventive Veterinary Medicine, 81(1–3), 92–116. https://doi.org/10.1016/j.prevetmed.2007.04.007
Woo, P. T. K., & Bruno, D. W. (Eds.). (2011). Fish diseases and disorders. Volume 3: Viral, bacterial and fungal infections (2nd ed.). CABI. https://doi.org/10.1079/9781845935542.0000
Zar, J. H. (1999). Biostatistical analysis (4th ed.). Prentice Hall.
Ziarati, M., Zorriehzahra, M. J., & Rezaei, H. (n.d.). Streptococcosis as an emerging bacterial disease in ornamental fish [Review].![]() |
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