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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 14, Issue:8, August, 2025

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2025.14(8): 32-43
DOI: https://doi.org/10.20546/ijcmas.2025.1408.004


Integrated Diagnostic Methodology for Identifying Bacterial, Parasitic and Fungal Pathogens in Goldfish (Carassius auratus)
Amreen Khan1*, Syed Atheruddin Quadri1 and Ishrat V. Shaikh2
1Department of Zoology, Maulana Azad College of Arts, Science and Commerce, Aurangabad, India 2Department of Zoology, Abeda Inamdar Senior College, Azam Campus, Pune, India
*Corresponding author
Abstract:

The ornamental fish trade has experienced rapid global growth, with goldfish (Carassius auratus) being one of the most widely traded species. However, high-density stocking, poor water quality, and transportation stress often predispose ornamental fishes to various infectious diseases, threatening their health and economic value. In this study, 65 naturally infected goldfish were collected from multiple aquarium shops in Aurangabad, Maharashtra, and examined using a multi-tiered diagnostic protocol. Parasitological investigations included wet mount examinations of gills, skin scrapings, and blood smears, which revealed the presence of common ectoparasites and Endoparasites. Bacteriological studies, including Gram staining, acid-fast staining, and biochemical assays (oxidase, catalase, TSI, citrate, motility, and indole tests), led to the identification of multiple fish-pathogenic bacteria. Fungal pathogens, primarily Saprolegnia spp., were identified based on characteristic cottony mycelial growth on infected skin and fins and confirmed using lactophenol cotton blue staining. Histopathological analysis of gill, liver, kidney, and skin tissues revealed cellular degeneration, hyperplasia, necrosis, and inflammatory infiltrates, which correlated with the severity of the infection. The integrated diagnostic approach employed in this study highlights the importance of early detection and accurate identification of pathogens in improving disease management strategies for ornamental fish culture. These findings provide a critical reference for developing prophylactic and therapeutic interventions in the Indian ornamental fish sector.


Keywords: Goldfish, Infectious Diseases, Bacteria, Parasites, Fungi


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How to cite this article:

Amreen Khan, Syed Atheruddin Quadri and Ishrat V. Shaikh. 2025. Integrated Diagnostic Methodology for Identifying Bacterial, Parasitic and Fungal Pathogens in Goldfish (Carassius auratus).Int.J.Curr.Microbiol.App.Sci. 14(8): 32-43. doi: https://doi.org/10.20546/ijcmas.2025.1408.004
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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