National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The rising demand of eco-efficient biomass conversion technology has given rise to the pursuance of strong microbial cellulases. Here we describe the molecular identification and enzymatic characterization of a very strong-actinobacterial cellulolytic organism, Streptomyces vinaceusdrappus strain AS14 that was isolated in environmental sources. Among 47 initial bacterial isolates tested on carboxymethyl cellulose (CMC) agar, AS14 displayed the highest hydrolysis index (HI = 7.68) that denotes the remarkable activity of extracellular cellulase. The molecular taxonomic identity based on 16S rRNA gene sequencing had shown it got the same relations with 96.72 similarity with S. vinaceusdrappus with strong bootstrap values in phylogenetic analysis. Analyzing functional assays indicated that the cellulase activity of AS14 is sensitive to environmental factors, with the best enzyme yield at the pH of 7.5 and 280C, which underpins the fact that the majority of the Streptomyces are mesophilic bacteria. The enzyme showed sharp decrease at above 450C and did not have activity at 600C implying thermolability. Such sensitivity makes AS14 a potential candidate in low-energy, ambient-temperature composting, bioremediation, and valorization of agricultural residues. Its long enzyme secretion, environmental versatility to pH and temperatures, and its close genetic sibling relationship with industrially proven Streptomyces strains makes it an attractive source of environmentally sustainable biotechnologies. AS14 can also play a huge role in waste-to-resource innovations in the circular bioeconomy context, with more optimization – either by fermentation strategies or metabolic engineering.
Boukaew, S., Prasertsan, P., Petlamul, W., & Cheirsilp, B. (2022). Palm oil decanter cake wastes as alternative nutrient sources for production of enzymes from Streptomyces philanthi RM-1-138 and the efficacy of its culture filtrate as an antimicrobial agent against plant pathogenic fungi and bacteria. Biomass Conversion and Biorefinery, 14(2), 1895–1904. https://doi.org/10.1007/s13399-022-02448-7
Celaya-Herrera, S., Casados-Vázquez, L. E., Valdez-Vazquez, I., Barona-Gómez, F., Bideshi, D. K., & Barboza-Corona, J. E. (2020). A Cellulolytic Streptomyces Sp. Isolated from a Highly Oligotrophic Niche Shows Potential for Hydrolyzing Agricultural Wastes. BioEnergy Research, 14(1), 333–343. https://doi.org/10.1007/s12155-020-10174-z
Fatokun, E., Nwodo, U., & Okoh, A. (2016). Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species. Applied Sciences, 6(10), 286. https://doi.org/10.3390/app6100286
Kurniawan, E., Panphon, S., & Leelakriangsak, M. (2019). Optimization of Chitinase Production by Bacillus sp. B26 and Its Antifungal Activity. Zenodo. https://doi.org/10.5281/zenodo.10056583
López-Reyes, P. K., De, S., María Mercedes Cortés-González, Galán-Wong, L. J., & Hamlet Avilés-Arnaut. (2024). Biological Control of Streptomyces sp. PR69 Against Phytophthora capsici and Its Growth-Promoting Effects on Plants. Horticulturae, 10(12), 1365–1365. https://doi.org/10.3390/horticulturae10121365
Maibeche, R., Nawel Boucherba, Kamel Bendjeddou, Prins, A., Cilia Bouiche, Hamma, S., Benhoula, M., Zahra Azzouz, Azzeddine Bettache, Said Benallaoua, & Marilize Le Roes-Hill. (2022). Peroxidase-producing actinobacteria from Algerian environments and insights from the genome sequence of peroxidase-producing Streptomyces sp. S19. International Microbiology, 25(2), 379–396. https://doi.org/10.1007/s10123-022-00236-x
Meenakshi, S., Hiremath, J., Meenakshi, M. H., & Shivaveerakumar, S. (2024). Actinomycetes: Isolation, Cultivation and its Active Biomolecules. Journal of Pure and Applied Microbiology, 18(1), 118–143. https://doi.org/10.22207/jpam.18.1.48
Mishra, P., Mishra, J., Dwivedi, S. K., & Arora, N. K. (2020). Microbial Enzymes in Biocontrol of Phytopathogens. Microorganisms for Sustainability, 259–285. https://doi.org/10.1007/978-981-15-1710-5_10
Naligama, K. N., Weerasinghe, K. E., & Halmillawewa, A. P. (2022). Characterization of Bioactive Actinomycetes Isolated from Kadolkele Mangrove Sediments, Sri Lanka. Polish Journal of Microbiology, 0(0). https://doi.org/10.33073/pjm-2022-017
Ocán-Torres, D., Walter José Martínez-Burgos, Maria Clara Manzoki, Vanete Thomaz Soccol, José, C., & Carlos Ricardo Soccol. (2024). Microbial Bioherbicides Based on Cell-Free Phytotoxic Metabolites: Analysis and Perspectives on Their Application in Weed Control as an Innovative Sustainable Solution. Plants, 13(14), 1996–1996. https://doi.org/10.3390/plants13141996
Passari, A. K., Chandra, P., Zothanpuia, Mishra, V. K., Leo, V. V., Gupta, V. K., Kumar, B., & Singh, B. P. (2016). Detection of biosynthetic gene and phytohormone production by endophytic actinobacteria associated with Solanum lycopersicum and their plant-growth-promoting effect. Research in Microbiology, 167(8), 692–705. https://doi.org/10.1016/j.resmic.2016.07.001
Rashikha A Siddiqui and S. A. Peshwe. (2019); Optimization Of Cultural And Nutritional Parameters For Enhanced Production Of Extracellular Laccase From Kalmusia SP RS07. Int. J. of Adv. Res. 7 (Mar). 773-781] (ISSN 2320-5407). www.journalijar.com
Sarkar, G., & Suthindhiran, K. (2022). Diversity and Biotechnological Potential of Marine Actinomycetes from India. Indian Journal of Microbiology. https://doi.org/10.1007/s12088-022-01024-x
Sinjaroonsak, S., Chaiyaso, T., & H-Kittikun, A. (2019). Optimization of Cellulase and Xylanase Productions by Streptomyces thermocoprophilus TC13W Using Low Cost Pretreated Oil Palm Empty Fruit Bunch. Waste and Biomass Valorization, 11(8), 3925–3936. https://doi.org/10.1007/s12649-019-00720-y
Waheeb, M. S., Elkhatib, W. F., Yassien, M. A., & Hassouna, N. A. (2024). Optimized production and characterization of a thermostable cellulase from Streptomyces thermodiastaticus strain. AMB Express, 14(1). https://doi.org/10.1186/s13568-024-01787-0
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