National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
To establish integrated colonisation of vectors in semi-insectary conditions. The pure strains of mosquitoes (Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus) were reared in similar semi- insectary/room conditions with exposure of natural light/darkness with front glass wall. One species of Sandfly (Phlebotomus argentipes) was reared inside the Biochemical Oxygen Demand (BOD) incubator at 26-280C, temperature and 72-80% relative humidity. Mosquito’s larvae were fed on yeast powder/fish food inside the tray having warm water at room temperature to avoid any contamination. Newly emerged adult females with male of An. stephensi and Cx. quinquefasciatus were exposed to a blood meal overnight and Ae. aegypti in day time. Fed females were kept in a cage with 10% glucose solution-soaked cotton ball. In respect of rearing of sandflies gravid females were confined in rearing pots. Larval food prepared with rabbit chow, faeces and sand was provided to the sandfly larvae in powder form. Newly emerged adults were exposed to blood meal for oviposition. Under limited resources and common room conditions mosquito life cycle was continued whole year (2023-2024) by maintaining continued generations of 10-15 days life cycle. Six generations of life cycles (28-36days) were maintained for sandflies. The life cycle of mosquito vector of different diseases can be reared together in similar semi-insectary conditions for the training/study/experiment purposes in limited resources and their life cycle can be maintained throughout the year.
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Puja T, Shakti KS, Anurag KK, Edgar R, David S, Om PS et al., (2017). Establishing, Expanding, and Certifying a Closed Colony of Phlebotomus argentipes (Diptera: Psychodidae) for Xenodiagnostic Studies at the Kala Azar Medical Research Center, Muzaffarpur, Bihar, India. J Med Entomol 2017; 54(5): 1129–1139. https://doi.org/10.1093/jme/tjx099
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