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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Drought is a major abiotic stress that reduces crop productivity and weaken the global food” security. Rice (Oryza sativa L.) is a semi-aquatic plant and it requires an adequate amount of water but drought has a detrimental effect on crop growth factors and caused a 25.4% decreased in rice output. It is therefore, imperative to understand the mechanism of plant responses to water deficit conditions and finding the rice germplasm that can withstand intermittent water stress in order to support crop development initiatives for periods of intermittent drought. The present investigation entitled “Unveiling The Hidden Half: Root Phenotyping of Rice for Enhanced Drought Tolerance under Rainfed Conditions” was carried out with the objective to quantify the variations existed among the genotypes. The planting materials used in the experiment consisted of 28 genotypes in which 27 rice genotypes obtained from IIRR, Hyderabad under the AICRP on rice program and 01 check variety from IGKV, Raipur. To understand the root dynamics, the root phenotyping study was conducted in rainout shelter of IGKV research farm using glass mini-rhizotron and root morphology scanning was done in Department of Plant Molecular Biology and Biotechnology lab. The root studied was carried out following the completely randomized design with three replication and two water treatments (watering regularly and watering after 2 days of interval). The field experiment was laid out in randomized block design with three replications under two water regimes (irrigated and rainfed) for two years (2020 and 2021) during kharif season. The results of analysis of variance revealed that the presence of greater diversity among the genotypes for all the observed root traits. Based on mean performance, the highest root length was observed in DT-127, genotype high total root length (DT-127), total root surface area (DT-127), root average diameter (DT-108), root volume (DT-127) and root anatomy (DT-122) in root phenotyping. Similarly, the genotypes were identified for higher total root length (DT-122), total root surface area (DT-122), root average diameter (DT-114), root volume (DT-111), root anatomy (DT-122) under rainfed conditions. The most of genotypes were showed variability across the genotypes for various attributes and genotypes were decreased under rainfed condition as compared to irrigated conditions. The variations among the genotypes and checks i.e., Indira Barani for each trait were noticed under irrigated and rainfed condition.
Araus, J.L., Kefauver, S.C., Zaman-Allah, M., Olsen, M.S. and Cairns, J.E., 2018. Translating high-throughput phenotyping into genetic gain. Trends in plant science, 23(5), pp.451-466. https://doi.org/10.1016/j.tplants.2018.02.001
Armengaud, P., Sulpice, R., Miller, A.J., Stitt, M., Amtmann, A. and Gibon, Y., 2009. Multilevel analysis of primary metabolism provides new insights into the role of potassium nutrition for glycolysis and nitrogen assimilation in Arabidopsis roots. Plant physiology, 150(2), pp.772-785. https://doi.org/10.1104/pp.108.133629
Atia, M.R., 2018. Investigation of ABS-rice straw composite feedstock filament for FDM. Rapid Prototyping Journal, 24(6), pp.1067-1075. https://doi.org/10.1108/RPJ-11-2017-0242
Bernier, J., Atlin, G.N., Serraj, R., Kumar, A. and Spaner, D., 2008. Breeding upland rice for drought resistance. Journal of the Science of Food and Agriculture, 88(6), pp.927-939. https://doi.org/10.1002/jsfa.3153
Bray, A.L. and Topp, C.N., 2018. The quantitative genetic control of root architecture in maize. Plant and Cell Physiology, 59(10), pp.1919-1930. https://doi.org/10.1093/pcp/pcy141
Bristiel, P., Roumet, C., Violle, C. and Volaire, F., 2019. Coping with drought: root trait variability within the perennial grass Dactylis glomerata captures a trade-off between dehydration avoidance and dehydration tolerance. Plant and Soil, 434, pp.327-342.
Cendrero-Mateo, M.P., Muller, O., Albrecht, H., Burkart, A., Gatzke, S., Janssen, B., Keller, B., Körber, N., Kraska, T., Matsubara, S. and Li, J., 2017. Field phenotyping: concepts and examples to quantify dynamic plant traits across scales in the field. In Terrestrial ecosystem research infrastructures (pp. 53-81). CRC Press.
Chaichi, M., Sanjarian, F., Razavi, K. and Gonzalez-Hernandez, J.L., 2019. Analysis of transcriptional responses in root tissue of bread wheat landrace (Triticum aestivum L.) reveals drought avoidance mechanisms under water scarcity. PloS one, 14(3), p.e0212671. https://doi.org/10.1371/journal.pone.0212671
Chen, R.K., Chen, K.Y., Liu, C.Y., Kao, S.M. and Chung, C.L., 2017. Genetic mapping of the qSBN7 locus, a QTL controlling secondary branch number per panicle in rice. Breeding science, 67(4), pp.340-347. http://doi.org/10.1270/jsbbs.17007
Chen, W., Gao, Y., Liu, X., Zhang, H., Xu, C., Yu, S., Zhang, Q. and Luo, J., 2013. Genetic analysis of the metabolome exemplified using a rice population. Proceedings of the National Academy of Sciences, 110(50), pp.20320-20325. https://doi.org/10.1073/pnas.1319681110
Comas, L.H., Becker, S.R., Cruz, V.M.V., Byrne, P.F. and Dierig, D.A., 2013. Root traits contributing to plant productivity under drought. Frontiers in plant science, 4, p.442. https://doi.org/10.3389/fpls.2013.00442
Courtois, B., Audebert, A., Dardou, A., Roques, S., Ghneim-Herrera, T., Droc, G., Frouin, J., Rouan, L., Gozé, E., Kilian, A. and Ahmadi, N., 2013. Genome-wide association mapping of root traits in a japonica rice panel. PloS one, 8(11), p.e78037. https://doi.org/10.1371/journal.pone.0078037
de Dorlodot, S., Forster, B., Pagès, L., Price, A., Tuberosa, R. and Draye, X., 2007. Root system architecture: opportunities and constraints for genetic improvement of crops. Trends in plant science, 12(10), pp.474-481. https://doi.org/10.1016/j.tplants.2007.08.012
Eshel, A. and Beeckman, T. eds., 2013. Plant roots: the hidden half. CRC press.
Ganapathy, S., Ganesh, S.K., Shanmugasundaram, P. and Babu, R.C., 2010. Studies on root traits for drought tolerance in rice (Oryza sative L.) under controlled (PVC pipes) condition. Electronic Journal of Plant Breeding, 1(4), pp.1016-1020.
Gowda, V.R., Henry, A., Yamauchi, A., Shashidhar, H.E. and Serraj, R., 2011. Root biology and genetic improvement for drought avoidance in rice. Field crops research, 122(1), pp.1-13. https://doi.org/10.1016/j.fcr.2011.03.001
Gu, D., Zhen, F., Hannaway, D.B., Zhu, Y., Liu, L., Cao, W. and Tang, L., 2017. Quantitative classification of rice (Oryza sativa L.) root length and diameter using image analysis. PloS one, 12(1), p.e0169968.
https://doi.org/10.1371/journal.pone.0169968
Guimarães, P.H.R., de Lima, I.P., de Castro, A.P., Lanna, A.C., Guimarães Santos Melo, P. and de Raïssac, M., 2020. Phenotyping root systems in a set of japonica rice accessions: can structural traits predict the response to drought?. Rice, 13, pp.1-19. https://doi.org/10.1186/s12284-020-00404-5
Han, E., Kautz, T. and Köpke, U., 2016. Precrop root system determines root diameter of subsequent crop. Biology and Fertility of Soils, 52, pp.113-118. http://dx.doi.org/10.1007/s00374-015-1049-5
Han, R., Khalid, M., Juan, J. and Huang, D., 2018. Exogenous glycine inhibits root elongation and reduces nitrate-N uptake in pak choi (Brassica campestris ssp. Chinensis L.). Plos one, 13(9), p.e0204488. https://doi.org/10.1371/journal.pone.0204488
Heinemann, A.B., Dingkuhn, M., Luquet, D., Combres, J.C. and Chapman, S., 2008. Characterization of drought stress environments for upland rice and maize in central Brazil. Euphytica, 162(3), pp.395-410. http://dx.doi.org/10.1007/s10681-007-9579-z
Henry, A., 2013. IRRI’s drought stress research in rice with emphasis on roots: accomplishments over the last 50 years. Plant Root, 7, pp.92-106. https://doi.org/10.3117/plantroot.7.92
Henry, A., Cal, A.J., Batoto, T.C., Torres, R.O. and Serraj, R., 2012. Root attributes affecting water uptake of rice (Oryza sativa) under drought. Journal of experimental botany, 63(13), pp.4751-4763. https://doi.org/10.1093/jxb/ers150
Jeong, J.S., Kim, Y.S., Baek, K.H., Jung, H., Ha, S.H., Do Choi, Y., Kim, M., Reuzeau, C. and Kim, J.K., 2010. Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions. Plant physiology, 153(1), pp.185-197. https://doi.org/10.1104/pp.110.154773
Jeong, J.S., Kim, Y.S., Redillas, M.C., Jang, G., Jung, H., Bang, S.W., Choi, Y.D., Ha, S.H., Reuzeau, C. and Kim, J.K., 2013. OsNAC5 overexpression enlarges root diameter in rice plants leading to enhanced drought tolerance and increased grain yield in the field. Plant Biotechnology Journal, 11(1), pp.101-114. https://doi.org/10.1111/pbi.12011
Kadam, N.N., Tamilselvan, A., Lawas, L.M., Quinones, C., Bahuguna, R.N., Thomson, M.J., Dingkuhn, M., Muthurajan, R., Struik, P.C., Yin, X. and Jagadish, S.K., 2017. Genetic control of plasticity in root morphology and anatomy of rice in response to water deficit. Plant physiology, 174(4), pp.2302-2315. https://doi.org/10.1104/pp.17.00500
Kano-Nakata, M., Gowda, V.R., Henry, A., Serraj, R., Inukai, Y., Fujita, D., Kobayashi, N., Suralta, R.R. and Yamauchi, A., 2013. Functional roles of the plasticity of root system development in biomass production and water uptake under rainfed lowland conditions. Field Crops Research, 144, pp.288-296. http://dx.doi.org/10.1016/j.fcr.2013.01.024
Kondo, Motohiko, P. P. Pablico, D. V. Aragones, R. Agbisit, Jun Abe, S. Morita, and Brigitte Courtois. "Genotypic and environmental variations in root morphology in rice genotypes under upland field conditions." In Roots: The Dynamic Interface between Plants and the Earth: The 6th Symposium of the International Society of Root Research, 11–15 November 2001, Nagoya, Japan, pp. 189-200. Springer Netherlands, 2003.
Kuijken, R.C., van Eeuwijk, F.A., Marcelis, L.F. and Bouwmeester, H.J., 2015. Root phenotyping: from component trait in the lab to breeding. Journal of experimental botany, 66(18), pp.5389-5401. https://doi.org/10.1093/jxb/erv239
Li, X., Guo, Z., Lv, Y., Cen, X., Ding, X., Wu, H., Li, X., Huang, J. and Xiong, L., 2017. Genetic control of the root system in rice under normal and drought stress conditions by genome-wide association study. PLoS Genetics, 13(7), p.e1006889. https://doi.org/10.1371/journal.pgen.1006889
Lynch, J.P., 2007. Roots of the second green revolution. Australian Journal of Botany, 55(5), pp.493-512. https://doi.org/10.1071/BT06118
Lynch, J.P., Chimungu, J.G. and Brown, K.M., 2014. Root anatomical phenes associated with water acquisition from drying soil: targets for crop improvement. Journal of Experimental Botany, 65(21), pp.6155-6166. https://doi.org/10.1093/jxb/eru162
Masuka, B., Araus, J.L., Das, B., Sonder, K. and Cairns, J.E., 2012. Phenotyping for abiotic stress tolerance in maize F. Journal of integrative plant biology, 54(4), pp.238-249. https://doi.org/10.1111/j.1744-7909.2012.01118.x
Matsui, T. and Singh, B.B., 2003. Root characteristics in cowpea related to drought tolerance at the seedling stage. Experimental Agriculture, 39(1), pp.29-38. https://doi.org/10.1017/S0014479703001108
Matthews, E., Fung, I. and Lerner, J., 1991. Methane emission from rice cultivation: Geographic and seasonal distribution of cultivated areas and emissions. Global Biogeochemical Cycles, 5(1), pp.3-24.
Mickelbart, M.V., Hasegawa, P.M. and Bailey-Serres, J., 2015. Genetic mechanisms of abiotic stress tolerance that translate to crop yield stability. Nature Reviews Genetics, 16(4), pp.237-251. https://doi.org/10.1038/nrg3901
Mir, R.R., Reynolds, M., Pinto, F., Khan, M.A. and Bhat, M.A., 2019. High-throughput phenotyping for crop improvement in the genomics era. Plant Science, 282, pp.60-72.
https://doi.org/10.1016/j.plantsci.2019.01.007
Paez-Garcia, A., Motes, C.M., Scheible, W.R., Chen, R., Blancaflor, E.B. and Monteros, M.J., 2015. Root traits and phenotyping strategies for plant improvement. Plants, 4(2), pp.334-355. https://doi.org/10.3390/plants4020334
Panda, S., 2017. Physiological impact of Zinc nanoparticle on germination of rice (Oryza sativa L) seed. J. Plant Sci. Phytopathol, 1, pp.062-070. https://dx.doi.org/10.29328/journal.jpsp.1001008
Passot, S., Moreno-Ortega, B., Moukouanga, D., Balsera, C., Guyomarc’h, S., Lucas, M., Lobet, G., Laplaze, L., Muller, B. and Guédon, Y., 2018. A new phenotyping pipeline reveals three types of lateral roots and a random branching pattern in two cereals. Plant physiology, 177(3), pp.896-910. https://doi.org/10.1104/pp.17.01648
Prathap, V., Ali, K., Singh, A., Vishwakarma, C., Krishnan, V., Chinnusamy, V. and Tyagi, A., 2019. Starch accumulation in rice grains subjected to drought during grain filling stage. Plant Physiology and Biochemistry, 142, pp.440-451. https://doi.org/10.1016/j.plaphy.2019.07.027
Price, A.H., Steele, K.A., Moore, B.J. and Jones, R.G.W., 2002. Upland rice grown in soil-filled chambers and exposed to contrasting water-deficit regimes: II. Mapping quantitative trait loci for root morphology and distribution. Field crops research, 76(1), pp.25-43. https://doi.org/10.1016/S0378-4290(02)00010-2
Ramanathan, V., Rahman, H., Subramanian, S., Nallathambi, J., Kaliyaperumal, A., Manickam, S., Ranganathan, C. and Muthurajan, R., 2018. OsARD4 encoding an acireductone dioxygenase improves root architecture in rice by promoting development of secondary roots. Scientific reports, 8(1), p.15713. https://doi.org/10.1038/s41598-018-34053-y
Ramireddy, E., Hosseini, S.A., Eggert, K., Gillandt, S., Gnad, H., von Wirén, N. and Schmülling, T., 2018. Root engineering in barley: increasing cytokinin degradation produces a larger root system, mineral enrichment in the shoot and improved drought tolerance. Plant physiology, 177(3), pp.1078-1095.
https://doi.org/10.1104/pp.18.00199
Sandhu, N., Subedi, S.R., Singh, V.K., Sinha, P., Kumar, S., Singh, S.P., Ghimire, S.K., Pandey, M., Yadaw, R.B., Varshney, R.K. and Kumar, A., 2019. Deciphering the genetic basis of root morphology, nutrient uptake, yield, and yield-related traits in rice under dry direct-seeded cultivation systems. Scientific reports, 9(1), p.9334. https://doi.org/10.1038/s41598-019-45770-3
Shashidhar, H.E., Henry, A. and Hardy, B. eds., 2012. Methodologies for root drought studies in rice. Int. Rice Res. Inst.
Shrestha, R., Al?Shugeairy, Z., Al?Ogaidi, F., Munasinghe, M., Radermacher, M., Vandenhirtz, J. and Price, A.H., 2014. Comparing simple root phenotyping methods on a core set of rice genotypes. Plant Biology, 16(3), pp.632-642. https://doi.org/10.1111/plb.12096
Sozzani, R., Busch, W., Spalding, E.P. and Benfey, P.N., 2014. Advanced imaging techniques for the study of plant growth and development. Trends in plant science, 19(5), pp.304-310. https://doi.org/10.1016/j.tplants.2013.12.003
Takehisa, H., Sato, Y., Igarashi, M., Abiko, T., Antonio, B.A., Kamatsuki, K., Minami, H., Namiki, N., Inukai, Y., Nakazono, M. and Nagamura, Y., 2012. Genome?wide transcriptome dissection of the rice root system: implications for developmental and physiological functions. The Plant Journal, 69(1), pp.126-140. https://doi.org/10.1111/j.1365-313x.2011.04777.x
Uga, Y., Kitomi, Y., Yamamoto, E., Kanno, N., Kawai, S., Mizubayashi, T. and Fukuoka, S., 2015. A QTL for root growth angle on rice chromosome 7 is involved in the genetic pathway of DEEPER ROOTING 1. Rice, 8, pp.1-8. https://doi.org/10.1186/s12284-015-0044-7
Wasaya, A., Zhang, X., Fang, Q. and Yan, Z., 2018. Root phenotyping for drought tolerance: a review. Agronomy, 8(11), p.241. https://doi.org/10.3390/agronomy8110241
Wasson, A.P., Richards, R.A., Chatrath, R., Misra, S.C., Prasad, S.S., Rebetzke, G.J., Kirkegaard, J.A., Christopher, J. and Watt, M., 2012. Traits and selection strategies to improve root systems and water uptake in water-limited wheat crops. Journal of experimental botany, 63(9), pp.3485-3498. https://doi.org/10.1093/jxb/ers111
Wullschleger, S.D. and Hanson, P.J., 2006. Sensitivity of canopy transpiration to altered precipitation in an upland oak forest: Evidence from a long?term field manipulation study. Global Change Biology, 12(1), pp.97-109.
Zhang, J., Zhang, S., Cheng, M., Jiang, H., Zhang, X., Peng, C., Lu, X., Zhang, M. and Jin, J., 2018. Effect of drought on agronomic traits of rice and wheat: A meta-analysis. International journal of environmental research and public health, 15(5), p.839. https://doi.org/10.3390/ijerph15050839
Zhu, G., Wang, S., Wang, Y., Wang, C., Risgaard-Petersen, N., Jetten, M.S. and Yin, C., 2011. Anaerobic ammonia oxidation in a fertilized paddy soil. The ISME Journal, 5(12), pp.1905-1912. http://dx.doi.org/10.1038/ismej.2011.63
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