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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 13, Issue:8, August, 2024

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2024.13(8): 85-90
DOI: https://doi.org/10.20546/ijcmas.2024.1308.010


A Unique Technique for Artificial Insemination in Tasar Silkworm, Antheraea mylitta with Fresh and Frozen Semen
Dinesh Kumar*, B. C. Prasad, A. K. Sinha, J. P. Pandey and P. K. Mishra
Silkworm Physiology Laboratory, Central Tasar Research and Training Institute, Central Silk Board, P.O. Piska Nagri, Ranchi-835 303, Jharkhand, India
*Corresponding author
Abstract:

Artificial insemination is a very important tool and has been used for hybrids, mutant lines and producing inbred lines is only possible with a method that ensures controlled mating. During the tasar eggs production, male and female emergence are differ as male moths abundance during early phase where as female later phase of grainage which resulted loss of uncoupled female moths (10 to 19%). To reduce loss of eggs and necessity of hybrid/inbred lines in Antheraea mylitta, present study was conducted. A unique and farmers friendly technique of artificial insemination of A. mylitta has been developed. A Tris buffer diluent with volume of 100 µl effective for homogenous mixing of semen collected from single male. The response was decreases on advancement of age of male and female for collection of semen and insemination. The data reveals that there were no significant difference observed among treatment on fecundity, hatching and chawki survival of larvae when female moth inseminated with fresh semen, semen kept at 0°C for 1 day and at -80°C for up to 120 days. It can be concluded that artificial insemination with fresh and frozen is feasible in A. myltta which is very helpful to tasar silk industry.


Keywords: Antheraea mylitta, tropical tasar silkworm, Artificial insemination, frozen semen


References:

Kumar, D., B. Subrahmanyam, S. K. Sharan, P. K. Mishra, B. M. K. Singh and N. Suryanarayana, 2008. Endogenous 20-hydroxyecdysone levels in the haemolymph of non-diapause-destined and diapause-destined generations of tasar silkworm, Antheraea mylitta (Lepidoptera: Saturniidae) and associated developmental changes. Eur. J. Entomol., 105: 591-598. https://doi.org/10.14411/eje.2008.079

Mochida, Y., Takemura, Y., Ohnuma, A., Kanekatsu, R. and Kiguchi, K. (2007). Fertilization of eggs developed from the cryopreserved and transplanted ovaries by artificial insemination with crypreserved semen in the silkworm, Bombyx mori. J. insect Biochem and Sericology, 78:97-100.

Moritz, R. F. A. (1984). The effect of different diluents on insemination success in the honeybee using mixed semen. J. Apic.Res. 23(3): 164-167.

Omura, S., 1938, Studies of the reproductive system of the male of Bombyx mori II, Post-testicular organs and post-testicular behaviour of the spermatozoa, J Fac Agr. Hokkaido Imperial University 40, 129-170.

Omura, S. 1936. Artificial insemination of Bombyx mori. J Fac Agr. Hokkaido Imperial University. 38:135-150.

Ruttner, F., 1975. - The instrumental insemination of the queen bee. Apimondia ublishing House, Bucharest.

Santhosh, H. T.  and Krishna, M. S.  (2013) Relationship between Male Age, Accessory Gland, Sperm Transferred, and Fitness Traits in Drosophila bipectinata. J Insect Sci. 2013; 13: 159. https://doi.org/10.1673/031.013.15901

Sturup M.  Baer-Imhoof, B.  Nash, D. R.  Boomsma, J. J. and Baer, B. (2013). When every sperm counts: factors affecting male fertility in the honeybee Apis mellifera. Behavioral Ecology (2013) https://doi.org/10.1093/beheco/art049.

Takemura Y, Kanda T, Horie Y. 1999. Artificial insemination using trypsin-treated sperm in the silkworm, Bombyx mori. J Insect Physiol. 45:471-477. https://doi.org/10.1016/s0022-1910(98)00148-6

Takemura Y, Kanda T, Horie Y. 2000. Artificial insemination using cryopreserved sperm in the silkworm, Bombyx mori. J Insect Physiol. 46:491-497. https://doi.org/10.1016/s0022-1910(99)00135-3

Takemura Y, Kanda T. 1999. Artificial insemination and oviposition using cryopreserved sperm in several strains of the silkworm, Bombyx mori. J Seric Sci Jpn. 68:327-332

Takemura, Y. Kanda, T, Tamura T, Shinbo H, Horie Y. 1996. Development of new method for artificial insemination of the silkworm, Bombyx mori. J Seric Sci Jpn 65:456-463.

Tobin P C, Bolyard J L, Onufrieva K S, Hickman A D. (2014). The effect of male and female age on Lymantria dispar (Lepidoptera: Lymantriidae) fecundity. J Econ Entomol. 107(3):1076-83. https://doi.org/10.1603/ec13561

Wang, X. P. Fang, Ling, Y. and Zhang, Z. N. (2011). Effects of delayed mating on the fecundity, fertility and longevity of females of diamondback moth, Plutella xylostella. Insect Science, 18(3):305–310 https://doi.org/10.1111/j.1744-7917.2010.01371.x  


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How to cite this article:

Dinesh Kumar, B. C. Prasad, A. K. Sinha, J. P. Pandey and Mishra, P. K. 2024. A Unique Technique for Artificial Insemination in Tasar Silkworm, Antheraea mylitta with Fresh and Frozen Semen.Int.J.Curr.Microbiol.App.Sci. 13(8): 85-90. doi: https://doi.org/10.20546/ijcmas.2024.1308.010
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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