National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Urinary tract infections has remained a public health challenge and Proteus species have been implicated as the second commonest cause after Escherichia coli and Klebsiella species. Proteus species belong to the family of Enterobacteriaceae. Pathogenicity of Proteus mirabilis is enhanced by their possession of unique virulence factors which it uses to colonize the host urinary tract, including urease and stone formation, adhesions/fimbria, toxins, and hemolysin. The aimed at identifying and characterizing Protues species from urine samples of patients attending Federal Medical Center Yenagoa (FMCY). A total of 50 urine samples were collected and analyzed using standard phenotypic and 16S rRNA amplification techniques. Of the 50 urine samples collected, 18(36%) were collected from males while 32(64%) from females. Four (4) bacterial isolates were analyzed by 16S rRNA gene sequencing, and the phylogenetic tree was constructed to ascertain Proteus species to its species level. The antimicrobial susceptibility pattern showed that out of the 7(14%) isolates of Proteus species 3(42.86%), 7(100%), 6(85.71%), 1(14.29%), 5(71.43%), 7(100%), 0, 0, 6(85.71%) and 2(28.57%) were resistant to (Sulfamethaxozole-trimethoprim), (Chloramphenicol), (Sparfloxacin), (Ciprofloxacin), Ampicillin, (Augmentin), (Gentamicin), Perfloxacin, (Tarivid), and (Streptomycin). Results from the study showed that the molecular characterization confirmed the identity of the organism as was obtained from the phenotypic characterization of the isolates. The study revealed widespread resistance to most routine antimicrobials, which calls for proper surveillance against Proteus species.
Adebo A., U. Umeh E., O. Ogbonna I (2023). Molecular Characterization of heavy metal resistant Proteus species. African Journal of Microbiology Research. 17(4):85-93. https://doi.org/10.5897/AJMR2022.9671
Kranz, J., Schmidt, S., Lebert, C., Schneidewind, L., Mandraka, F., and Kunze, M. (2018). The 2017 update of the German clinical guideline on epidemiology, diagnostics, therapy, prevention, and management of uncomplicated urinary tract infections in adult patients. Part II: therapy and prevention. UrolInt, 100(3), 271- 278. https://doi.org/10.1159/000487645
Chelsie E. Armbruster., Harry L. T. Mobly., Melanie M. Pearson (2018). Pathogenesis of Proteus mirabilis Infection. Ecosal Plus. https://doi.org/10.1128/ecosalplus.ESP-0009-2017
Enaam Badeh Faleh (2011). Pathological Changes of Immunized Rabbits with Proteus vulgarius fimbriae antigen. The Iraqi Journal of Veterinary Medicine. https://doi.org/10.30539/iraqijvm.v35i2.584
Majeed, H. T., and Aljanaby, A. J. (2019). Antibiotic Susceptibility Patterns and Prevalence of Some Extended Spectrum Beta-Lactamases Genes in Gram-Negative Bacteria Isolated from Patients Infected with Urinary Tract Infections in Al-Najaf City, Iraq. Avicenna Journal of Medicine & Biotechnology,11(2), 192-201.
Md. Mahabubul Islam Majumder., Tarek Ahmed., Saled Ahmed., Ashiqur Rahman Khan (2018). Microbiology of Catheter Associated Urinary Tract Infection, Intech Open. https://doi.org/10.5772/intechopen.80080
Meini, S., Tascini, C., Cei, M., Sozio, E., and Rossolini, G. M. (2019). AmpC β-lactamase-producing Enterobacterales: what a clinician should know. Infection, 47(3), 363-375. https://doi.org/10.1007/s15010-019-01291-9
Meumann E. M., Mitchell B. G., McGregor A., McBryde E., and Cooley, L. (2015). Urinary Escherichia coli antimicrobial susceptibility profiles and their relationship with community antibiotic use in Tasmania, Australia. International Journal of Antimicrobial Agents,46(4), 389-393. https://doi.org/10.1016/j.ijantimicag.2015.05.015
Nakashige, T. G., Zygiel, E. M., Drennan, C. L., and Nolan, E. M. (2017). Nickel sequestration by the host-defense protein human calprotectin. Journal of American Chemical Sociology, 139(26), 8828-8836. https://doi.org/10.1021/jacs.7b01212
Norsworthy, A. N., and Pearson, M. M. (2017). From catheter to kidney stone: the uropathogenic lifestyle of Proteus mirabilis. Trends in Microbiology, 25(4), 304-315. https://doi.org/10.1016/j.tim.2016.11.015
Odoki, M., Aliero, A., Tibyangye, J., Nyabayo, J., Wampande, E., Drago Kato, C., Agwu, E., and Bazira, J. (2019). Prevalence of Bacterial Urinary Tract Infections and Associated Factors among Patients Attending Hospitals in Bushenyi District, Uganda. International Journal of Microbiology, 4(2), 467-480. https://doi.org/10.1155/2019/4246780
O'Keefe, L. C., Koelle, P., McGee, Z., Dewberry, L. S., Wright, C., Stallings, J. E., Gates, E., and Chittur, K. (2019). Innovations in Worksite Diagnosis of Urinary Tract Infections and the Occupational Health Nurse. Workplace Health Safety, 67(6), 268-274. https://doi.org/10.1177/2165079919834310
Pavez, M., Troncoso, C., Osses, I., Salazar, R., Illesca, V., Reydet, P., Rodríguez, C… and Barrientos, L. (2019). High prevalence of CTX-M-1group in ESBL-producing Enterobacteriaceae infection in intensive care units in southern Chile. Brazilian Journal of Infectious Disease, 23(2), 102-110. https://doi.org/10.1016/j.bjid.2019.03.002
Pelling, H., Nzakizwanayo, J., Milo, S., Denham, E. L., MacFarlane, W. M., Bock, L. J., Sutton, J. M., and Jones B. V. (2019). Bacterial biofilm formation on indwelling urethral catheters. Letter Applied Microbiology, 68(4), 277-293. https://doi.org/10.1111/lam.13144
Potron, A., Hocquet, D., Triponney, P., Plésiat, P., Bertrand, X., and Valot, B. (2019). Carbapenem-Susceptible OXA-23-Producing Proteus mirabilis in the French Community. Antimicrob Agents Chemother, 63(6), 12-19. https://doi.org/10.1128/AAC.00191-19
Slattery, S., Tony, Pembroke, J., Murnane, J. G., and Ryan, M. P. (2020). Isolation, nucleotide sequencing and genomic comparison of a Novel SXT/R391 ICE mobile genetic element isolated from a municipal wastewater environment. Science Report,10, 871-876. https://doi.org/10.1038/s41598-020-65216-5
Sun, L., Xu, J., and He, F. (2019). Genomic characterization of a Proteus mirabilis clinical isolate from China carrying blaNDM-5 on an IncX3 plasmid. Journal of Global Antimicrobiology Resistance,19, 317–319. https://doi.org/10.1016/j.jgar.2019.10.025
Wang, S., Zhang, Y., Zhang, X., and Li, J. (2020). An evaluation of multidrug-resistant (MDR) bacteria in patients with urinary stone disease: data from a high-volume stone management center. World Journal Urology,38(2), 425-432. https://doi.org/10.1007/s00345-019-02772-0
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