Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Login as a Reviewer


See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 13, Issue:3, March, 2024

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2024.13(3): 139-165
DOI: https://doi.org/10.20546/ijcmas.2024.1303.014


Bacterial Profile of Urinary Tract Infection and Antimicrobial Susceptibility Pattern among the Patients attending a Tertiary Care Centre
S. Kowsalya and C. Meghna*
PIMS, Kalapet, PIMS Hospital, Ganapathy Chatticulam, India
*Corresponding author
Abstract:

Urinary Tract Infection (UTI) is a most cause of health issues in the world. It is the major cause of morbidity and mostly caused by bacterial infections from children to adults estimating the prevalence of UTI. To study the bacterial profile of urinary tract infection among the patients attending in a tertiary care center and also to assess the antimicrobial susceptibility patterns of the bacterial isolates. This study was conducted in the Pondicherry Institute of Medical Science Hospital, Pondicherry from 1st December 2020 to 30th January 2021. A total of 200 midstream urine samples were collected from suspected UTI patients attending in tertiary care settings. Urine samples were inoculated and the isolates were identified according to the standard microbiological techniques. The antimicrobial susceptibility tests were performed by Kirby-Bauer disk diffusion method on Mueller-Hinton agar according to the CLSI guidelines. Out of 200 samples, 107 positive urine cultures, UTI caused more in females (56%) when compared to males (44%). The most common bacteria frequently identified in this study were E.coli (57%) and followed by (12.15%) from gram negative isolates while Enterococcus species (7.48%) were common pathogen in gram positive organisms. The gram negative isolated were >70% sensitive to Amikacin, Nitrofurantoin and Piperacillintazobactam while >70% resistant to ampicillin, Nitrofloxacin and Cefazolin. The Gram positive isolates were 100% sensitive Vancomycin and Linezolid. The multi-drug resistance (MDR) was seen in most of the isolates. This study found a number of bacterial isolates with very high resistance to the commonly prescribed drugs from the patients with and without symptoms of UTI.


Keywords: Urinary Tract Infection (UTI), E.coli, Klebsiella pneumoniae, Pseudomonas aeruginosa


References:

Abujnah AA, Zorgani A, Sabri MA, El-Mohammady H, Khalek RA, Ghenghesh KS. Multidrug resistance and extended-spectrum β-lactamases genes among Escherichia coli from patients with urinary tract infections in Northwestern Libya. Libyan J Med. 2015;10:26412. https://doi.org/10.3402/ljm.v10.26412

Ana L. Flores-Mireles, Jennifer N. Walker, Michael Caparon, Scott J. Hultgren. 2015 Urinary tract infections: epidemiology, mechanisms of infection and treatment options, Nat Rev Microbiol. Author manuscript; available in PMC 2016 May 1. Published in final edited form as: Nat Rev Microbiol. 2015 May; 13(5): 269–284. https://doi.org/10.1038/nrmicro3432,  

Ashenafi Tula, Abraham Mikru, TsegayeAlemayehu, BeyeneDobo, "Bacterial Profile and Antibiotic Susceptibility Pattern of Urinary Tract Infection among Pregnant Women Attending Antenatal Care at a Tertiary Care Hospital in Southern Ethiopia", Canadian Journal of Infectious Diseases and Medical Microbiology, vol. 2020, Article ID 5321276, 9 pages, 2020. https://doi.org/10.1155/2020/5321276

Bency JAT, Priyanka R, Jose P. A study on the bacteriological profile of urinary tract infection in adults and their antibiotic sensitivity pattern in a tertiary care hospital in central Kerala, India. Int J Res Med Sci 2017;5:666-9. https://doi.org/10.18203/2320-6012.ijrms20170171

Chee Wei Tan, Maciej Piotr Chlebicki. Urinary tract infections in adults, Singapore Med J. 2016 Sep; 57(9): 485–490. https://doi.org/10.11622/smedj.2016153   

Das UK, Bhattacharjee P, Debnath S, Chakraborty M, Ghosh R, Das l, et al., Antibiotic sensitivity pattern of bacterial isolates from urine samples of admitted patients with urinary tract infection in a tertiary care teaching hospital of Tripura, India: a hospital record based study. Int J Basic ClinPharmcol 2018; 7:585-9. https://doi.org/10.18203/2319-2003.ijbcp20181013

Dave V R, Shah V R, Sonaliya K N, Shah S D, Gohel A R. A Study on Epidemiological Profile of Urinary Tract Infections in Perspective of Diabetic Status among Patients Attending Tertiary Care Hospital, Ahmedabad. Natl J Community Med 2018;9(8):594-598  

Debolina Chatterjee1, Sarthak Kumar Sen2. Bacteriological Profile And Antibiotic Susceptibility Pattern of Urine Samples Among Adult Patients Attending The Outpatient Department At A Tertiary Care Hospital In Kolkata. International Journal of Scientific & Engineering Research Volume 9, Issue 7, July-2018 ISSN 2229-5518

Derese B, Kedir H, Teklemariam Z, Weldegebreal F, Balakrishnan S. Bacterial profile of urinary tract infection and antimicrobial susceptibility pattern among pregnant women attending at Antenatal Clinic in DilChora Referral Hospital, Dire Dawa, Eastern Ethiopia. TherClin Risk Manag. 2016;12:251-260 https://doi.org/10.2147/TCRM.S99831

Dnyaneshwari Puroshottam Ghadage, Vrishali Avinash Muley, Jyotika Sharma, Arvind Vamanrao Bhore . Bacteriological Profile and Antibiogram of Urinary Tract Infections at a Tertiary Care Hospital https://doi.org/10.7860/NJLM/2016/22026:2164

Fenta, A., Dadnew, M., Eshetie, S. et al., Bacterial profile, antibiotic Susceptibility pattern and associated risk factors of urinary tract infection among clinically suspected children Attending at Felege-Hiwot comprehensive and Specialized hospital, Northwest Ethiopia. BMC Infect Dis 20, 673 (2020). https://doi.org/10.1186/s12879-020-05402-y

Gharavi, M. J., Zarei, J., Roshani-Asl, P. et al., Comprehensive study of antimicrobial susceptibility pattern and extended spectrum beta-lactamase (ESBL) prevalence in bacteria isolated from urine samples. Sci Rep 11, 578 (2021). https://doi.org/10.1038/s41598-020-79791-0

Kalita D1, Deka S2, Sarma R3, Rasul ES4, Talukdar B5, Hazarika NK6. Bacteriological profile and drug-resistance in Urinary Tract Infection from a rural area of Northeast India. Received on April 24, 2016; editorial approval on June 12, 2016

Lautika Sonkar1, Rampal Singh2, Imran Ali3, Ved Prakash4, DeepikaVerma. Antimicrobial Susceptibility Pattern of Various Etiological Agents Causing Pediatric Urinary Tract Infection. DOI: http://dx.doi.org/10.21276/ijcmr.2020.7.10.23

Mohammed MA, Alnour TM, Shakurfo OM, Aburass MM. Prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in Messalata Central Hospital, Libya. Asian Pac J Trop Med. 2016;9(8):771-6.  https://doi.org/10.1016/j.apjtm.2016.06.011

Manandhar, R., Raghubanshi, B., Mahato, M., Neupane, S., & Lama, R. (2020). Bacteriological Profile and Antimicrobial Susceptibility Patterns of Urine Culture Isolates from Patients in a Tertiary Care Centre in Lalitpur. Birat Journal of Health Sciences, 5(1), 881-885. https://doi.org/10.3126/bjhs.v5i1.29602

Martha Medina and Edgardo Castillo-Pino (2019). An introduction to the epidemiology and burden of urinary tract infections TherAdv Urol. 2019 Jan-Dec; 11: 1756287219832172. Published online 2019 May 2. https://doi.org/10.1177/1756287219832172  

Michel Kengne, AmonTodjimbaideDounia, Julius MbekemNwobegahay . Bacteriological profile and antimicrobial susceptibility patterns of urine culture isolates from patients in Ndjamena, Chad Pan Afr Med J. 2017; 28: 258. Published online 2017 Nov 23. https://doi.org/10.11604/pamj.2017.28.258.11197  

Mohamed Abdel-Aziz Elzayat, Ashton Barnett-Vanes, Mohamed FaragElmorsyDabour, Feng Cheng (2017). Prevalence of undiagnosed asymptomatic bacteriuria and associated risk factors during pregnancy: a cross-sectional study at two tertiary centres in Cairo, Egypt https://doi.org/10.1136/bmjopen-2016-013198

National Institute of Diabetes and Digestive and Kidney Diseases, National Kidney and Urologic Diseases Clearinghouse. (2011). Urinary tract infections in adults.   

National Institute on Aging. (2011). Urinary incontinence. Retrieved May 16, 2012, from  

Nure Ishrat Nazme1, Abdullah Alamin1, Farhana Jalil2, Jesmin Sultana3, Nurun Nahar Fatema4. Bacteriological Profile of Urinary Tract Infection in Children of a Tertiary Care Hospital bangladesh J Child Health 2017; Vol 41 (2): 77-83 https://doi.org/10.3329/bjch.v41i2.36102

Pegu B, Gaur BPS, Bora I et. al. Detection of urinary tract infection among pregnant women in a tertiary care hospital. Int J Health Sci Res. 2014;4(7):83-88.

Pezzani M. D., Antinori S. (2018) Introduction to Urinary Tract Infections: An Overview on Epidemiology, Risk Factors, Microbiology and Treatment Options. In: Tonolini M. (eds) Imaging and Intervention in Urinary Tract Infections and Urosepsis. Springer, Cham. https://doi.org/10.1007/978-3-319-68276-1_2

Pharsuram Adhikari1 and Dharma Prasad Khanal Antibiotic Resistance Patterns and Evaluation of Treatment of Inpatient with Urinary Tract Infections in Two Tertiary Care Hospitals at Kathmandu Valley. Journal of Pharmacy Practice and Community Medicine.2020, http://dx.doi.org/10.5530/jppcm.2020.1.4

Sabih A and Leslie S W. Complicated Urinary Tract Infections. (Updated 2021 Feb 10). In: StatPearls (Internet). Treasure Island (FL): StatPearls Publishing; 2021.  

Salwa H Almukhtar, (2018) Urinary Tract Infection Among Women Aged (18-40) Years Old in Kirkuk City, Iraq Department of Clinical Nursing Sciences, College of Nursing, University of Mosul, Mosul, Iraq,  https://doi.org/10.2174/1874434601812010248

Shailaja T S and Mohankumar A. Bacteriological profile of urinary tract infection in a tertiary care centre. Indian J Microbiol Res 2017; 4(3):328-332. https://doi.org/10.18231/2394-5478.2017.0072

Shamim, R. and Ramalakshmi Sathiss. (2018) Bacterial Profile and Antimicrobial Susceptibility Pattern in Pregnant Women with Urinary Tract Infection Attending the Department of OBG in a Tertiary Care Hospital, https://dx.doi.org/10.22207/JPAM.12.2.61

Sharma, A, S Shrestha, S Upadhyay and P Rijal. Clinical and Bacteriological profile of urinary tract infection in children at Nepal Medical College Teaching Hospital Nepal Med Coll J 2011; 13(1): 24-26

Shashi Kant, AyushLohiya,, Sanjeev Kumar Gupta. Urinary tract infection among pregnant women at a secondary level hospital in Northern India. Year : 2017 | Volume : 61 | Issue : 2 118-123. https://doi.org/10.4103/ijph.IJPH_293_15

Sneka P, Mangayarkarasi V. Bacterial pathogens causing UTI and their antibiotic sensitivity pattern: a study from a tertiary care hospital from South India. Trop J Path Micro 2019;5(6):379-385. https://doi.org/10.17511/jopm.2019.i6.08.

Thass N, Kumar M, Kaur R, Prevalence and antibiogram of bacterial pathogen causing urinary tract infection in a tertiary care hospital. Int J Med Sci Public Health 2019;8(1):53-57. https://doi.org/10.5455/ijmsph.2019.0926517102018

Timothy O O, Olusesan F J, Adesola B O, Temitayo A A, David F O, Ige O O. Antibiotic Resistance Pattern of bacterial isolates from cases of urinary tract infections among hospitalized and out-patients at a tertiary health facility in South Western Nigeria. Ann Trop Med Public Health 2014;7;130-5. https://doi.org/10.4103/1755-6783.146403

Urvashi Chongtham, ChitraYengkokpam, H. Lokhendro. “Bacterial Uropathogens in Urinary Tract Infections and Antibiotic Susceptibility Pattern of Patients Attending Jnims Hospital, Imphal”. Journal of Evolution of Medical and Dental Sciences 2013; Vol2, Issue 50, December 16; Page: 9769-9774.  https://doi.org/10.14260/jemds/1691

Yonas Alem Gessese, Dereje Leta Damessa Mebratenesh Mengistu Amare1, Yonas Hailesilassie Bahta, Assalif Demisew Shifera, Fikreslasie Samuel Tasew and Endrias Zewdu Gebremedhin Gessese et al., Urinary pathogenic bacterial profile, antibiogram of isolates and associated risk factors among pregnant women in Ambo town, Central Ethiopia: A cross-sectional study. Antimicrobial Resistance and Infection Control (2017) 6:132 https://doi.org/10.1186/s13756-017-0289-6



Download this article as Download

How to cite this article:

Kowsalya, S. and Meghna, C. 2024. Bacterial Profile of Urinary Tract Infection and Antimicrobial Susceptibility Pattern Among the Patients Attending A Tertiary Care Centre.Int.J.Curr.Microbiol.App.Sci. 13(3): 139-165. doi: https://doi.org/10.20546/ijcmas.2024.1303.014
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

Citations