Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 1105-1123
DOI: https://doi.org/10.20546/ijcmas.2017.605.120


Secondary Metabolites Approach to Study the Bio-Efficacy of Trichoderma asperellum Isolates in India
N. Srinivasa1*, S. Sriram2, Chandu Singh3 and K.S. Shivashankar2
1Division of Plant Pathology, ICAR-Indian Agricultural Research Institute (IARI),Pusa campus, New Delhi-11012, India
2Division of Plant Pathology and Physiology, ICAR-Indian Institute of Horticultural Research, Bangalore 560089, India
3Seed Production Unit, ICAR-Indian Agricultural Research Institute (IARI), Pusa campus,New Delhi-11012, India
*Corresponding author
Abstract:

10 isolates of Trichoderma asperellum was used for characterization of secondary metabolites through gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) analysis to establish valid correlation between the production of antifungal metabolites and their bio-efficacy as BCAs. The investigation revealed that the culture filtrate of T. asperellum isolates were showed the presence of 673 secondary metabolites at different retention time with a range of 39 (Ta-20) to 101 (Ta-12) with GC-MS. Out 673 volatile metabolites, 55 metabolites were found to be most abundant from which seven metabolites from Ta-14 and Ta-20, six metabolites from Ta-8, Ta-17 and Ta-29, five metabolites from Ta-45, Ta-15, Ta-10 and Ta-12 and remaining three metabolites from Ta-2 isolate respectively. Further, the five isolates viz.,Ta-2, Ta-8, Ta-10, Ta-20 and Ta-45 were used for the LC-MS and study showed the presence of nine antifungal metabolites viz., Viridin, Viridiol, Butenolides, Harzianolides, Ferulic acid, Viridiofungin A, Cyclonerodiol, Massoilactone and Gliovirin. Hence, these isolates were produced highest number of major volatile and antimicrobial compounds. Therefore, these isolates viz., Ta-45, Ta-10, Ta-20, Ta-8, and Ta-2 were considered as high potential bio-control agents against Sclerotium rolfsii pathogens.


Keywords: Trichoderma asperellum, Metabolomics, secondary metabolites, antifungal compounds, GC-MS, LC-MS, Sclerotium rolfsii, retention time.
Download this article as Download

How to cite this article:

Srinivasa, N., S. Sriram, Chandu Singh and Shivashankar, K.S. 2017. Secondary Metabolites Approach to Study the Bio-Efficacy of Trichoderma asperellum Isolates in India.Int.J.Curr.Microbiol.App.Sci. 6(5): 1105-1123. doi: https://doi.org/10.20546/ijcmas.2017.605.120