National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Aim of this study was to investigate the relationship between some kinematic parameters of ram semen and reproductive performance of dairy sheep after application of assisted reproductive technologies. The experiment was carried out with8 rams and 325 sheep from East Friesian breed and 7 rams and 162 sheep from Asaaf breed. Semen was collected by artificial vagina and subjected to primary assessment. Kinematic sperm parameters TM, PM, NPM, IS, VAP, VCL, VSL, LIN and STR were evaluated by computer-assisted semen analysis (CASA). All sheep were subjected to estrus synchronization by intravaginal sponges and artificially inseminated byfresh-diluted semen. Reproductive performance was recorded on the base of pregnancy rate (PR) and average litter size (ALS) calculated after lambing. Relationships between sperm kinematic parameters and these with the reproductive prformance were determined by correlation analysis. Significant differences between the mean values of age, body weight, ejaculate volume and sperm concentrationin the rams, and PR and ALS were not found. The sperm kinematic parametersshowed variability between individual rams. Total motility correlated positively with PM and LIN andnegativelywith NPM and IS (P<0.05). The correlation of PM with LIN and STR was positive, while this with NPM, IM and VCL was negative (P<0.05). A negative relationshipwas found of NPP and IS with STR and between LIN and VCL(P<0.05). The relationship of PM, VAP, LIN, STR with PR, and of LIN and STR with ALS (P<0.05) was positive, whereas this between VCL with PR was negative (P<0.05). In conclusion, the evaluation of the kinematic parameters by CASA is useful diagnostic and prognostic tool in sheep reproduction. The parameters PM, VAP, LIN and STR have a strong positive relationship (P<0.05) with the functional activity of ram spermatozoa and the reproductive performance of the sheep. They could be included as indicators in evaluation of the fresh semen quality in a selection of East Friesian and Asaaf rams with high fertility.
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