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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 12, Issue:9, September, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(9): 194-204
DOI: https://doi.org/10.20546/ijcmas.2023.1209.019


Isolation, Characterization of Azotobacter and PSB from Xerophytic Plants of Maharashtra State, India
Monika Suresh Barge, T. K. Narute, A. M. Navale, D. R. Murumkar,A. G. Durgude, P. H. Gaikwad, Satyam and S. A. Shewale
Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri – 413722, India
*Corresponding author
Abstract:

The present study was conducted at Department of Plant Pathology and Agricultural Microbiology, MPKV, Rahuri during year 2021 to 2023 to characterize and screen different isolates of Azotobacter and PSB from Different Xerophytic plants from Different districts of Maharashtra State.68 Azotobacter isolates were biochemically characterized and screened under In- vitro conditions for their plant growth promoting properties. The highly efficient Azotobacter isolates was characterized by its nitrogenase activity, which was 189.4 nmol C2H4. mg protein-1.h-1 in Azt 2 (Zizipus spp) isolates, Out of sixty eight Azotobacter isolates, 3 isolates viz., Azt- 2 (Ziziphus spp), Azt-17 (Aloe spp), Azt- 8 (Agave spp), recorded significantly higher nitrogenase activity. The amount of Pi released from tri- calcium phosphate (TCP) by the isolates at 10 day after inoculation was ranged from 18.5 to 29.65 %. The quantitative estimation of per cent inorganic phosphorus released from Tri- calcium phosphate had recorded highest for PSB-4 (Zizipus spp) which was 29.65 % Thus, isolate PSB-4 (Zizipus spp) i.e. efficient strain isolated from ber was found to be significantly superior over rest of the isolate in solubilizing phosphate.


Keywords: Azotobacter, PSB, Xerophytic plant, Acetylene Reduction Assay

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How to cite this article:

Monika Suresh Barge, T. K. Narute, A. M. Navale, D. R. Murumkar, A. G. Durgude, P. H. Gaikwad, Satyam and Shewale, S. A. 2023. Isolation, Characterization of Azotobacter and PSB from Xerophytic Plants of Maharashtra State, India.Int.J.Curr.Microbiol.App.Sci. 12(9): 194-204. doi: https://doi.org/10.20546/ijcmas.2023.1209.019
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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