National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Among various industrial enzymes, lipases have an important role in a wide variety of industrial applications. There are very few reports, that lipase was obtained from Lysinibacillus fusiformis L-52 bacteria isolated from dairy industry effluents. This Extracellular Lipoprotein Lipase (ELPL) released from the isolated bacteria was purified partially by using ammonium sulfate precipitation and Sephadex G-150 column chromatography. The purified enzyme was found to be a lipid-containing protein with a molecular weight of 69.89kDa approximately. It has shown the lipolytic activity on various substrates, specifically on natural substrates like olive oil and sunflower oil, and on synthetic substrate 4-para Nitro Phenyl Palmitate (pNPP). ELPL showed a Km value of 0.045mM with pNPP as substrate and has an optimum temperature at 370C, and optimum pH of 7.0. The enzyme was found to be active in hydrolyzing dairy effluent and also serum lipids. ELPL also showed a significant effect in hydrolyzing plant-based lipids containing monounsaturated fatty acids like oleic acid and linoleic acid but not on animal fats like butter.
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