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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
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Original Research Articles                      Volume : 12, Issue:9, September, 2023
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
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Int.J.Curr.Microbiol.App.Sci.2023.12(9): 95-104
DOI: https://doi.org/10.20546/ijcmas.2023.1209.009


Exploitation of 16s rRNA Gene as a Tool for Identification of Pseudomonas syringae pv. tagetis, the Causal Agent of Bacterial Leaf Spot Disease of French Marigold
Pinky Gurjar* and Laxmi Kant Sharma
Department of Botany, Raj Rishi Govt. College, Alwar, (Raj.), India
*Corresponding author
Abstract:

The aim of this study was to isolate, identify Pseudomonas syringae pv. tagetis (Pst) from seeds of French marigold (Tagetes patula). A total of 25 bacterial pathogens were isolated and tested for basic biochemical and host tests. Results of basic biochemical and host tests primarily indicated the presence of Pseudomonas syringae pv. tagetis. The 16S rRNA gene sequencing was performed on PCR amplified product with 8F and 1492R primers. The two isolates Tp-03 and Tp-09 showing 99.34% and 99.20%, sequence homology with Pst strain LF2012 which was isolated from seeds of French marigold.


Keywords: 16s rRNA sequencing, Bacterial leaf spot

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How to cite this article:

Pinky Gurjar and Laxmi Kant Sharma. 2023. Exploitation of 16s rRNA Gene as a Tool for Identification of Pseudomonas syringae pv. tagetis, the Causal Agent of Bacterial Leaf Spot Disease of French Marigold.Int.J.Curr.Microbiol.App.Sci. 12(9): 95-104. doi: https://doi.org/10.20546/ijcmas.2023.1209.009
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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