Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 892-902
DOI: https://doi.org/10.20546/ijcmas.2017.605.099


Influence of Storage Duration and Storage Temperature on In-Vitro Pollen Germination of Citrus Species
Shahnawaz Ahmed1*, H.S. Rattanpal1, Eajaz Ahmad3 and Gurteg Singh1
1Division of Fruit Science, PAU, Ludhiana, Punjab – 141004, India
3Department of Agronomy, Ludhiana, Punjab – 141004, India
*Corresponding author
Abstract:

The aim of present study was to access the pollen in- vitro germination of pollen grains of five citrus species belonging to the family Rutaceae viz., mandarin (citrus. reticulata Blanco) sweet orange (C. sinensis L. Osbeck) grapefruit (C. paradisi Macf.) pummelo (C. maxima Burm. Merr.) and tangelo (C. reticulata × C. maxima or × C. paradise) under low temperature storage conditions during year 2014. Pollen germination were checked up to 48 weeks, stored at different temperatures like 4ºC, -20ºC, and -196ºC. Pollen stored at low temperature (-196℃ and -20℃) showed better germination percentage as compared to pollen stored at 40C and room temperature. The cultivar Mosambi had maximum average germination percentage when pollens were stored at -196℃ (70.8 %,) -20℃ (66.3 %,) which was significantly higher than all other genotypes and followed by W.Murcott -196℃ (60.9%) -20℃ (48.5%). Minimum germination percentage was found in Marsh Seedless stored at -196℃ (21.7%) -20℃ (12.5%). The results indicate that pollen collected and stored at sub-zero temperatures from early blooming citrus varieties can be stored for very long period without any appreciable loss of germination. The stored pollen can be used along the whole blooming season for hybridization programmes by fruit breeders.


Keywords: Citrus, In- vitro, Pollen, Storage, Temperature, Species and Germination.
Download this article as Download

How to cite this article:

Shahnawaz Ahmed, H.S. Rattanpal, Eajaz Ahmad and Gurteg Singh. 2017. Influence of Storage Duration and Storage Temperature on In- Vitro Pollen Germination of Citrus Species.Int.J.Curr.Microbiol.App.Sci. 6(5): 892-902. doi: https://doi.org/10.20546/ijcmas.2017.605.099