National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study aimed to compare the Rapid card test with Enzyme linked Immunosorbent assay and to detect the seroprevalence of anti- HCV infection in Greater Noida. Hepatitis C Virus (HCV) belongs to family Flaviviridae and genus Hepacivirus. It is a single stranded, enveloped, positive sense RNA virus.Liver is the main site of infection and is transmitted via- blood and blood. This study was conducted to compared between a Rapid card test and Enzyme linked Immunosorbent Assay (ELISA) for detecting Hepatitis C Virus antibody in a human serum. The present study was conducted in the Serology section Department of Microbiology, School of Medical Sciences & Research, Sharda University for a period of 12 months (1st Nov 2020-31st Oct 2021) to comparative evaluate the detection of Hepatitis C Virus antibodies by rapid card test and ELISA. A total of 386 samples were taken for the test. Out of 386 samples, only a total of 92 samples were selected randomly for ELISA test with 46 negatives and other 46 positives samples from rapid test. Out of 46 negatives sample by rapid 1 turns out to be positive and from the 46 positive ICT samples 5 turns out to be negative by ELISA. The sensitivity and specificity of ICT was 90% and 97.61% respectively the PPV was 97.82% and the NPV was 91.30% when compare with ELISA. The seroprevalence was found to be 1.72%. Hence, anti HCV screening can be preferably done by Rapid Card test followed by a supplemental ELISA and Polymerase Chain Reaction for further confirmation.
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