National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Proteases are an important class of enzymes with numerous industrial applications. Under submerged cultivation conditions, the Bacillus subtilis MH266414 generated alkaline protease. In order to maximise the factors such as pH, incubation period, and temperature, response surface methodology-based central composite design (CCD) was utilised. The quadratic model was shown to be reasonably adjusted with the experimental data by the CCD design. To assess the changes in the response surface and to understand the connection between the culture conditions and the enzyme yield, statistics-based contour and 3-D plots were created. In this investigation, a highest yield of protease activity of 63.0725 U/mL were obtained after optimization. The crude enzyme was purified using ammonium sulphate precipitation and DEAE Sephadex column chromatography. The fraction obtained from the column shows a specific activity of 3512U/mg-1. The isolated protease also gives better result of washing and dehairing results. This result implies that this purified enzyme from Bacillus subtilis MH266414 can be used as biotechnological tool for various industries.
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