International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 12, Issue:1, January, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(1): 153-158

Pupal Dimorphic Coloration is Sex-Specific in Harmonia axyridis - Kuwait
Department of Science, College of Basic Education, Public Authority for Applied Education and Training, Building 10, Office 36, Ardyia, Safat - 23167, Kuwait
*Corresponding author

This study is the first to use pupal pigmentation of the ladybug H. axyridis, a species from Kuwait, to identify sex prior to adult emergence. Twenty adults were collected from fields and reared under laboratory-controlled measures. Based on preliminary observations, it was hypothesized that orange pupae would develop to females, whereas males would emerge from dark pupae. 100 orange & 100 dark brown were collected from the second generation of the reared colony and each reared under similar laboratory conditions. Ninety-seven orange pupae developed into females, whereas 94 dark brown pupae developed into males. Another two hundred pupae were collected randomly from the field, each in a separate Petri-dish, and were left to grow in the field. Ninety-six percent of orange pupae developed into females, whereas ninety-nine percent of males developed dark brown pupae. For validation, the mean total area of melanin pigmentation present on the dorsal surface of pupae was measured using Image-J software and the results revealed significant variations for both field and laboratory samples of different colors. The overall outcome was consistent with the proposition that the pupal dimorphic colorsare sex-specific and could be applied to identify the sex prior to adult emergences in H. axyridis.

Keywords: Pupae, laboratory, ladybird, temperature, sex, adult, larvae, pigmentation, melanin

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How to cite this article:

Jenan Salem Alharbi and Nadia Abdulaziz Alhajeri. 2023. Pupal Dimorphic Coloration is Sex-Specific in Harmonia axyridis - Kuwait.Int.J.Curr.Microbiol.App.Sci. 12(1): 153-158. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.