International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 12, Issue:1, January, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(1): 99-104

Isolation, Screening and Characterization of Polyhydroxybutyrate (PHB) Producing Bacteria from Landfill Soil
Nandini Prajapati, Hetvi Rami and Nimisha Patel*
Parul Institute of Applied Sciences and Research (PIASR), Parul University, P.O. Ghuma,
Bopal-Ghuma road, Ahmedabad, Gujarat-380058, India
*Corresponding author

Today, environment is polluted with waste which is not degradable. Waste placed at landfill dumping site which contain plastics account about 20% by volume of municipal solid wastes and reduce the capacity of precious landfill sites. Poly hydroxyl butyrate (PHB) which is a biodegradable and biocompatible thermoplastic compound has broadly similar physical properties to poly (propylene). It has many applications in medicine, veterinary practice, tissue engineering materials, food packaging and agriculture due to its biodegradability. In the present study, we focus on screening of bioplastics producing bacterial isolates from the soil sample from dumping site. The bacteria were characterized by their cultural characteristics, morphology and biochemical test. PHB producing bacterial isolate was further detected by using Sudan black- B staining reagent. Attempt was made to produce and extract PHB by using our bacterial isolate. Isolate exhibited significant PHB yields, thus showing a potential for further exploitation. Further analyses are currently ongoing to try to extract and characterize PHB granules.

Keywords: Bioplastic, Bacillus sp., Biodegradation, PHB producers

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How to cite this article:

Nandini Prajapati, Hetvi Rami and Nimisha Patel. 2023. Isolation, Screening and Characterization of Polyhydroxybutyrate (PHB) Producing Bacteria from Landfill Soil.Int.J.Curr.Microbiol.App.Sci. 12(1): 99-104. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.