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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(5): 722-728
DOI: https://doi.org/10.20546/ijcmas.2017.605.081


In vitro Mass Propagation of Endangered Terrestrial Orchid Phaius tankervilliae (L’Her.) Blume through Green Seed Pod Culture
Rocky Thokchom*, Soumen Maitra and Sachin Sharma
Department of Floriculture, Medicinal and Aromatic Plants, Faculty of Horticulture, Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar – 736165, West Bengal, India
*Corresponding author
Abstract:

Phaius tankervilliae (L’Her.) Blume is one of the most attractive and horticulturally valuable ground orchids of the world. Due to illegal collection for trade and consumption and loss of habitat, the population of this species has decreased rapidly at an alarming rate in natural habitats. In vitro seed germination and seedling development of Phaius tankervilliae technique was successfully established for rapid multiplication using 0.8% (w/v) agar solidified MS medium supplemented with different concentrations and combinations of Kinetin (Kin) and NAA. MS medium supplemented with 1.0 mg L¬¬-1 Kin + 1.0 mg L¬¬-1 NAA was the most ideal condition for early seed germination (2.87 weeks), fastest protocorm formation (5.07 weeks), tallest shoots (6.85 cm) and maximum number of roots per plant (6.20). However, maximum number of shoots (52.27) per culture and maximum number of shoots with roots (50.27) were produced when the plantlets were transferred to MS basal medium supplemented with 1.5 mg L¬¬-1 Kin + 1.0 mg L¬¬-1 NAA. The well developed in vitro rooted plantlets were hardened successfully in the potting mixture containing only vermiculite (86.00 %) followed by vermiculite + vermicompost (71.00 %) at 1:1 ratio.


Keywords: Phaius tankervilliae, in vitro, seed germination, Kinetin, NAA
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How to cite this article:

Rocky Thokchom, Soumen Maitra and Sachin Sharma. 2017. In vitro Mass Propagation of Endangered Terrestrial Orchid - Phaius tankervilliae (L’Her.) Blume through Green Seed Pod CultureInt.J.Curr.Microbiol.App.Sci. 6(5): 722-728. doi: https://doi.org/10.20546/ijcmas.2017.605.081