National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of the study was to investigate the antibacterial activity of aqueous leaf extract of E. crassipes against fish pathogenic organisms like P.aeruginosa, A.hydrophila, Vibrio sp., E. coli and S.aureus. O.mosambicus was collected from Kolathur area, Chennai. Fishes were packed aseptically and transported to lab. Organisms were isolated and identified from fishes and used for further studies. The aqueous leaf extract of E. crassipes was extracted by using Soxhlet apparatus and concentrated by Vacuum evaporator to remove the moisture. The phytochemical screening was done to show that the aqueous leaf extract of E. crassipes contain alkaloids, sterols, flavonoids, carbohydrates, and terpenoids, anthoquinones and proteins. The occurrence of these biologically active compounds of the plants may justify their wide usage in traditional medicine and effective against various disease causing pathogens. TLC, HPTLC and GC-MS were performed to study the qualitative and quantitative analysis of various compounds present in the plant extract. Due to the presence of these phytochemical compounds, the leaves can be used as a natural source to treat against the selected fish pathogenic organisms. MIC and the antibacterial activity of the aqueous plant extract against the selected pathogens were done by well diffusion method. The diameters of growth inhibition zone formed were measured in mm along with tetracycline as a reference standard.
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