International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 11, Issue:11, November, 2022

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(11): 103-112

In silico allergenicity evaluation of cry proteins of Bacillus thuringiensis expressed in Bt-Brinjal (Solanum melongena L.)
Sujit Kumar Das1,2, Sukanta Kumar Pradhan2, Kailash Chandra Samal3 and Nihar Ranjan Singh4*
1Department of Botany, Ravenshaw University, Cuttack-753003, Odisha, India
2Department of Bioinformatics, Centre for Post Graduate Studies,
3Department of Agricultural Biotechnology, College of Agriculture, Odisha University of Agriculture & Technology, Bhubaneswar-751003, Odisha, India
4Department of Botany and Centre of Excellence in Environment and Public Health, Ravenshaw University, Cuttack-753003, Odisha, India
*Corresponding author

Prior to commercial production, it is necessary to assess the biosafety of the novel recombinant proteins added to Bt-Brinjal (Bacillus thuringiensis) with regard to their probable allergenicity. In the Bt-brinjal plant, the cry1Ac, cry1Ab, and cry1Fa1 genes produce toxic proteins that are highly lethal to insects, particularly lepidopteron insects, and offer defence against the fruit and shoot borer (FSB), also known as Leucinodes orbonalis. However these proteins are inactivated in acidic environment of the human and animal gut, so they are not toxic. In the current study, cry1Ac, cry1Ab, and cry1Fa1 proteins produced in Bt-Brinjal were assessed for their probable allergenic cross-reactivity by sequence comparison using bioinformatics techniques. In silico assessment, these recombinant proteins expressed in Bt-Brinjal were compared for homology using online databases (Allergen Online version 21.0, AlgPred, and SDAP), BLASTX program of NCBI and FASTA. Sequence alignment with allergen proteins was performed using three approaches: complete sequence, 80 amino acids, and 6-8 amino acids. In addition, the cry proteins were examined for the existence of domains that matched those of the allergen proteins to determine whether allergen cross-reactivity was likely even in the absence of sequence homology. Sequence alignment using the three approaches above and at the epitope level shows no similarity of cry1Ac, cry1Ab, and cry1Fa1 to any of the known allergen proteins. Since the potential cross-reactivity of these recombinant proteins with allergen proteins was not reached, this result may help farmers, researchers, and policy makers to promote the cultivation of Bt-brinjal in terms of biosafety.

Keywords: In silico, Bacillus thuringiensis, Allergen, Bt-brinjal, Bioinformatics, Genetically modified plants, Nihar Singh

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How to cite this article:

Sujit Kumar Das, Sukanta Kumar Pradhan, Kailash Chandra Samal and Nihar Ranjan Singh. 2022. In silico allergenicity evaluation of cry proteins of Bacillus thuringiensis expressed in Bt-Brinjal (Solanum melongena L.).Int.J.Curr.Microbiol.App.Sci. 11(11): 103-112. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.