National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The Heat shock proteins (HSP) play an important role in controlling the apoptosis and inflammatory response. These proteins are released into the extracellular environment and act as mediators of inflammation. Protein folding accuracy is ensured by chaperone activity, which also prevents the accumulation of abnormal isoforms. In the current work, Sartoriana spinigera, an endemic freshwater crab, is examined for its survival and Hsps expression patterns in ovarian tissues in response to short-term heat stress. Crabs maintained at 25 oC served as control. Expression of Hsp genes were analyzed using semi-quantitative and quantitative PCR in both groups to see whether these crabs have lost the Hsp gene plasticity. Hsp gene expression analysis showed up-regulation of hsp90 (2.1-fold) and hsp47 (2.5-fold) in heat stressed crabs, whereas there was no alteration in expression of other Hsps. Up-regulation of all the Hsps was observed in the heat shocked crabs, whereas expression of all Hsps were down regulated to the basal level in crabs maintained at 34 oC/24 hour. These results indicate that Hsp90 and Hsp60 play important role in survival of Sartoriana spinigera under thermal stress.
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