International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 11, Issue:9, September, 2022

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(9): 113-119

Stability Characteristics of Freeze-Dried IVRI-CSF-BS Classical Swine Fever Vaccine
Chayna Singha Mahapatra, Aruna Kuniyal, Shyma K. Latheef and Pronab Dhar*
Department of Biological Standardization, ICAR-Indian Veterinary Research Institute,
Izatnagar, 243122, Uttar Pradesh, India
*Corresponding author

Freeze-drying (lyophilization) of biologically active materials including attenuated live virus vaccine is an well accepted method for ensuring stability and preserving the vaccine potency. As freeze- drying does not ensure complete stability, studies for stability of freeze-dried vaccines after exposure at different temperatures are required. Heat stability of classical swine fever (CSF) virus vaccine is also urgently needed in tropical countries including India where the disease is still endemic. In this study, the thermostability of freeze- dried vials of live attenuated CSF vaccines were investigated at 4℃ and 25℃ for 30 days. This lyophilized vaccine containing combination of sucrose, lactose and lactalbumen hydrolysate gives good heat stability and viral infectivity was reduced by a factor of 0.2 to 1 Log10TCID50/mL at the end of freeze drying. In this experiment, we have studied storage stability of lyophilized classical swine fever vaccine produced at Division of Biological Standardization, IVRI using IVRI-CSF-BS vaccine strain which can be stored at -20℃ for more than a year without any loss. Stability at 4℃ and 25℃ may ensure easy transportation and minimize expense related to that.

Keywords: Live attenuated classical swine fever, stabilizer, lyophilization, thermostability, vaccine titre

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How to cite this article:

Chayna Singha Mahapatra, Aruna Kuniyal, Shyma K. Latheef and Pronab Dhar. 2022. Stability Characteristics of Freeze-Dried IVRI-CSF-BS Classical Swine Fever Vaccine.Int.J.Curr.Microbiol.App.Sci. 11(9): 113-119. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.