Non-fermenting gram negative bacilli (NFGNB) are a group of aerobic, non spore forming bacilli. They either do not use carbohydrates as a source of energy or degrade them through metabolic pathways other than fermentation. They are ubiquitous in nature. Although they are commonly considered to be environmental contaminants, they have emerged as important nosocomial pathogens. Aim of this study was to characterize the prevalence of NFGNB distribution from various clinical isolates and to evaluate their antibiotic sensitivity patterns. Material and methods: A total 11,040 various clinical specimen were received in bacteriology laboratory, Department of Microbiology at Kamineni Institute of Medical Sciences. Non fermenters are identified and further analysed as per the guidelines. Antimicrobial susceptibility testing was performed by Kirby beaur disc diffusion method. Results: Among 11,040 clinical samples 354 yields NFGNB. Pseudomonas species (63.55%) and Acinetobacter species (32.20%) were the most commonly isolated NFGNB. A high level of antibiotic resistance was recorded. Ciprofloxacin (71.2) and Gentamicin (54.33) were the drugs with maximum activity. Conclusion: Identification of NFGNB and monitoring their antimicrobial susceptibility pattern helps in proper management of the treatment.

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