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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(12): 276-282
DOI: https://doi.org/10.20546/ijcmas.2021.1012.032


Isolation of Causal Organism of Wilt and Collar Rot of Lentil and its Pathogenicity Tests
Abhishek Rathod1, Babli1, Shivangi Tiwari2, Dharmendra Mishra3, Ravi Patel3,Kamlesh Choubey3, Jyoti Kurmi2 and Dwarka4*
1Department of Plant Pathology, Rajmata Vijayaraje Scindhia Krishi Vishva Vidyalaya, Gwalior, Madhya Pradesh, India
2Eklavya University, Damoh, Madhya Pradesh, India
3Swami Vivekanand University, Sagar, Madhya Pradesh, India
4Department of Entomology, Jawahar Lal Nehru Krishi Vishwa Vidyalaya, Jabalpur,
Madhya Pradesh, India
*Corresponding author
Abstract:

The disease appears in the field in patches at both seedling and adult stages. Seedling wilt is characterized by sudden drooping, followed by drying of leaves and seedling death. The roots appear healthy, with reduced proliferation and nodulation and usually no internal discoloration of the vascular system. The fungus Fusarium oxysporum f. sp. lentis is septate, profusely branched growth on potato sucrose / dextrose agar at 25ºC, initially white turning light buff or deep brown later and fluffy or submerged. The growth becomes felted or wrinkled in old cultures. Various types of pigmentation (yellow, brown, whitish /cream, crimson dark purple, light orange) may be observed in culture on solid medium. Microconidia were usually borne on simple and short conidiophores, which arise laterally on the hyphae. They are oval to cylindrical, straight or curved and measure 2.5 - 3.5 x 5 - 11µm. Macroconidia are borne on branched conidiophore, thin walled, 1 to 6 septate, fusoid, pointed at both ends and measures 3.5 -4.5 x 25 - 65µm. Chlamydospores are formed in old cultures, which are smooth or rough walled, terminal intercalary and may be formed singly or in pairs or in chains. The pathogen causes a lesion localized at or about the collar between the stem and the root. The lesions develop around the stem eventually forming a "collar" region.


Keywords: Lentil, chlamydospores, growth, discoloration and infecting
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How to cite this article:

Abhishek Rathod, Babli, Shivangi Tiwari, Dharmendra Mishra, Ravi Patel, Kamlesh Choubey, Jyoti Kurmi and Dwarka. 2021. Isolation of Causal Organism of Wilt and Collar Rot of Lentil and its Pathogenicity Tests.Int.J.Curr.Microbiol.App.Sci. 10(12): 276-282. doi: https://doi.org/10.20546/ijcmas.2021.1012.032